Nanoparticles (NPs) represent one of the most promising tools for drug-targeting and drug-delivery. However, a deeper understanding of the complex dynamics that happen after their in vivo administration is required. Particularly, plasma proteins tend to associate to NPs, forming a new surface named the 'protein corona' (PC). This surface is the most exposed as the 'visible side' of NPs and therefore, can have a strong impact on NP biodistribution, targeting efficacy and also toxicity. The PC consists of two poorly delimited layers, known as 'hard corona' (HC) and 'soft corona' (SC), that are affected by the complexity of the environment and the formed protein-surface equilibrium during in vivo blood circulation. The HC corona is formed by proteins strongly associated to the NPs, while the SC is an outer layer consisting of loosely bound proteins. Several studies attempted to investigate the HC, which is easier to be isolated, but yielded poor reproducibility, due to varying experimental conditions. As a consequence, full mapping of the HC for different NPs is still lacking. Moreover, the current knowledge on the SC, which may play a major role in the 'first' interaction of NPs once in vivo, is very limited, mainly due to the difficulties in preserving it after purification. Therefore, multi-disciplinary approaches leading to the obtainment of a major number of information about the PC and its properties is strongly needed to fully understand its impact and to better support a more safety and conscious application of nanotechnology in medicine.

Protein corona and nanoparticles: How can we investigate on? / Pederzoli, Francesca; Tosi, Giovanni; Vandelli, Maria Angela; Belletti, Daniela; Forni, Flavio; Ruozi, Barbara. - In: WILEY INTERDISCIPLINARY REVIEWS. NANOMEDICINE AND NANOBIOTECHNOLOGY. - ISSN 1939-5116. - (2017), pp. e1467-e1467. [10.1002/wnan.1467]

Protein corona and nanoparticles: How can we investigate on?

PEDERZOLI, FRANCESCA;TOSI, Giovanni;VANDELLI, Maria Angela;BELLETTI, Daniela;FORNI, Flavio;RUOZI, Barbara
2017

Abstract

Nanoparticles (NPs) represent one of the most promising tools for drug-targeting and drug-delivery. However, a deeper understanding of the complex dynamics that happen after their in vivo administration is required. Particularly, plasma proteins tend to associate to NPs, forming a new surface named the 'protein corona' (PC). This surface is the most exposed as the 'visible side' of NPs and therefore, can have a strong impact on NP biodistribution, targeting efficacy and also toxicity. The PC consists of two poorly delimited layers, known as 'hard corona' (HC) and 'soft corona' (SC), that are affected by the complexity of the environment and the formed protein-surface equilibrium during in vivo blood circulation. The HC corona is formed by proteins strongly associated to the NPs, while the SC is an outer layer consisting of loosely bound proteins. Several studies attempted to investigate the HC, which is easier to be isolated, but yielded poor reproducibility, due to varying experimental conditions. As a consequence, full mapping of the HC for different NPs is still lacking. Moreover, the current knowledge on the SC, which may play a major role in the 'first' interaction of NPs once in vivo, is very limited, mainly due to the difficulties in preserving it after purification. Therefore, multi-disciplinary approaches leading to the obtainment of a major number of information about the PC and its properties is strongly needed to fully understand its impact and to better support a more safety and conscious application of nanotechnology in medicine.
15-mar-2017
e1467
e1467
Protein corona and nanoparticles: How can we investigate on? / Pederzoli, Francesca; Tosi, Giovanni; Vandelli, Maria Angela; Belletti, Daniela; Forni, Flavio; Ruozi, Barbara. - In: WILEY INTERDISCIPLINARY REVIEWS. NANOMEDICINE AND NANOBIOTECHNOLOGY. - ISSN 1939-5116. - (2017), pp. e1467-e1467. [10.1002/wnan.1467]
Pederzoli, Francesca; Tosi, Giovanni; Vandelli, Maria Angela; Belletti, Daniela; Forni, Flavio; Ruozi, Barbara
File in questo prodotto:
File Dimensione Formato  
WNAN_1467.pdf

accesso aperto

Tipologia: Pre-print dell'autore (bozza pre referaggio)
Dimensione 2.03 MB
Formato Adobe PDF
2.03 MB Adobe PDF Visualizza/Apri
pederzoli2017.pdf

non disponibili

Tipologia: Versione dell'editore (versione pubblicata)
Dimensione 477.19 kB
Formato Adobe PDF
477.19 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1131695
Citazioni
  • ???jsp.display-item.citation.pmc??? 16
  • Scopus 81
  • ???jsp.display-item.citation.isi??? 66
social impact