To contribute to the undecided question if a retrovirus of the human T-cell lymphotropic virus (HTLV) family may be involved in the development of multiple sclerosis (MS), we investigated by the polymerase chain reaction (PCR) the presence of HTLV-I and HTLV-II sequences in the peripheral blood mononuclear cell DNAs from 30 patients affected by MS and 15 by chronic progressive myelopathy. Moreover a control group of 14 blood donors was examined. All these patients were devoid of anti-HTLV-I antibody in the serum and cerebrospinal fluid at ELISA. For the PCR, primers and probes, specific for the tax region common to HTLV-I and HTLV-II, for the pol region of HTLV-I, and for the pol region of HTLV-II were used. In spite of the high sensitivity of the technique used, the three groups of subjects were negative for HTLV-I and HTLV-II genomic sequences.
FAILURE TO DETECT GENOMIC MATERIAL OF HTLV-I OR HTLV-II IN MONONUCLEAR-CELLS OF ITALIAN PATIENTS WITH MULTIPLE-SCLEROSIS AND CHRONIC PROGRESSIVE MYELOPATHY / Merelli, E; Sola, P; Marasca, Roberto; Salati, R; Torelli, Giuseppe. - In: EUROPEAN NEUROLOGY. - ISSN 0014-3022. - STAMPA. - 33:(1993), pp. 23-26.
FAILURE TO DETECT GENOMIC MATERIAL OF HTLV-I OR HTLV-II IN MONONUCLEAR-CELLS OF ITALIAN PATIENTS WITH MULTIPLE-SCLEROSIS AND CHRONIC PROGRESSIVE MYELOPATHY
MARASCA, Roberto;TORELLI, Giuseppe
1993
Abstract
To contribute to the undecided question if a retrovirus of the human T-cell lymphotropic virus (HTLV) family may be involved in the development of multiple sclerosis (MS), we investigated by the polymerase chain reaction (PCR) the presence of HTLV-I and HTLV-II sequences in the peripheral blood mononuclear cell DNAs from 30 patients affected by MS and 15 by chronic progressive myelopathy. Moreover a control group of 14 blood donors was examined. All these patients were devoid of anti-HTLV-I antibody in the serum and cerebrospinal fluid at ELISA. For the PCR, primers and probes, specific for the tax region common to HTLV-I and HTLV-II, for the pol region of HTLV-I, and for the pol region of HTLV-II were used. In spite of the high sensitivity of the technique used, the three groups of subjects were negative for HTLV-I and HTLV-II genomic sequences.Pubblicazioni consigliate
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