Scientific question and context The role of diet in shaping the gut microbiota has been evaluated in different animal models, including insects. Drosophila flies host an inconstant microbiota, among which acetic acid bacteria (AAB) are important components, capable of modulating the immune response and insect development. Here, we characterized the bacterial and AAB communities associated to the spotted wing fly Drosophila suzukii, an invasive pest, by studying the same insect population separately grown on two different food sources, i.e. on fruit‐based or non‐fruit artificial diets. Methods By the use of a specific PCR the prevalence of AAB has been investigated in the two insect populations. AAB capability to colonize the gut has been estimated by fluorescent in situ hybridization (FISH) and recolonization experiments with green fluorescent protein (Gfp)‐labelled strains. The bacterial communities of individuals reared on the two diets have been evaluated by 16S rRNA gene pyrosequencing, analysing the V1‐V3 region of the 16S rRNA gene. Results AAB were highly prevalent in the two insect populations with infection rates of 90 and 92% in fruit‐fed and artificial diet‐fed individuals, respectively. FISH and recolonization experiments with Gfp‐labelled strains showed AAB capability to massively colonize the insect gut. 16S rRNA gene pyrosequencing showed a differentiation of the bacterial microbiota of guts from insects fed with the two diets. The exclusion of AAB‐ related OTUs from the analysis showed a lack of the clustering pattern, suggesting that the diet‐based diversification of the community is primarily reflected on AAB components. Diet influenced also AAB alpha‐ diversity. Conclusion High prevalence, localization and massive recolonization, together with AAB clustering behaviour in relation to diet, suggest an AAB role in the gut microbiota response of D. suzukii to diet modification.
|Data di pubblicazione:||2017|
|Titolo:||Bacterial diversity shift determined by different diets in the gut of the spotted wing fly Drosophila suzukii is primarily reflected on acetic acid bacteria|
|Autore/i:||Crotti, E; Vacchini, V; Gonella, E; Prosdocimi, Em; Mazzetto, F; Chouaia, B; Callegari, F; Mapelli, F; Mandrioli, Mauro; Alma, A; Daffonchio, D.|
|Nome del convegno:||4th Symposium on Bacterial Genetics and Ecology|
|Data del convegno:||4-8 giugno 2017|
|Luogo del convegno:||Aberdeen, Scotland.|
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