We recently described a bioluminescence in vivo imaging technique, representing a powerful tool to test the real-time progression of oropharyngeal candidiasis, hence potentially useful to evaluate the efficacy of antifungal therapies. In this study, the in vivo imaging technique was compared with CFU measurement of target organs (tongue, esophagus and stomach) for monitoring and quantifying oropharyngeal candidiasis. We have correlated these two analytical methods at different times post-infection using engineered, luminescent Candida albicans in mice rendered susceptible to oral candidiasis by cortisone-acetate. Scatter plots, Pearson correlation and Student's t test were used to compare the methods. We observed that the bioluminescence in vivo imaging technique was more reliable than CFU counts in detecting early infection of, and its extent in, the oral cavity of the mouse. This was also evident following the introduction of a variable such as treatment with fluconazole. The results described in this study could validate the bioluminescence in vivo imaging technique as a method to monitor and quantify oropharyngeal candidiasis and to assess early discovery of active compounds in vivo.

Comparison between bioluminescence imaging technique and CFU count for the study of oropharyngeal candidiasis in mice / Gabrielli, E; Roselletti, E; Luciano, E; Sabbatini, S; Mosci, P; Pericolini, Eva. - In: CYTOMETRY. PART A. - ISSN 1552-4922. - 87A:5(2015), pp. 428-436. [10.1002/cyto.a.22666]

Comparison between bioluminescence imaging technique and CFU count for the study of oropharyngeal candidiasis in mice

PERICOLINI, Eva
2015

Abstract

We recently described a bioluminescence in vivo imaging technique, representing a powerful tool to test the real-time progression of oropharyngeal candidiasis, hence potentially useful to evaluate the efficacy of antifungal therapies. In this study, the in vivo imaging technique was compared with CFU measurement of target organs (tongue, esophagus and stomach) for monitoring and quantifying oropharyngeal candidiasis. We have correlated these two analytical methods at different times post-infection using engineered, luminescent Candida albicans in mice rendered susceptible to oral candidiasis by cortisone-acetate. Scatter plots, Pearson correlation and Student's t test were used to compare the methods. We observed that the bioluminescence in vivo imaging technique was more reliable than CFU counts in detecting early infection of, and its extent in, the oral cavity of the mouse. This was also evident following the introduction of a variable such as treatment with fluconazole. The results described in this study could validate the bioluminescence in vivo imaging technique as a method to monitor and quantify oropharyngeal candidiasis and to assess early discovery of active compounds in vivo.
26-mar-2015
87A
5
428
436
Comparison between bioluminescence imaging technique and CFU count for the study of oropharyngeal candidiasis in mice / Gabrielli, E; Roselletti, E; Luciano, E; Sabbatini, S; Mosci, P; Pericolini, Eva. - In: CYTOMETRY. PART A. - ISSN 1552-4922. - 87A:5(2015), pp. 428-436. [10.1002/cyto.a.22666]
Gabrielli, E; Roselletti, E; Luciano, E; Sabbatini, S; Mosci, P; Pericolini, Eva
File in questo prodotto:
File Dimensione Formato  
Gabrielli E. et al Cytometry A 2015.pdf

non disponibili

Descrizione: Gabrielli E. et al, Cytometry A, 2015
Tipologia: Versione dell'editore (versione pubblicata)
Dimensione 552.82 kB
Formato Adobe PDF
552.82 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/1120022
Citazioni
  • ???jsp.display-item.citation.pmc??? 8
  • Scopus 9
  • ???jsp.display-item.citation.isi??? 9
social impact