EnglishThe lipophilic cation JC-1 (5,5',6,6'-tetrachloro-1,1',3,3'-etraethylbenzimidazolyl carbocyanine iodide) has been used for more than 20 years as a specific dye for measuring mitochondrial membrane potential (δψm). In this unit, we revise our original protocol (that made use of a single 488 nm laser for the detection of monomers and aggregates, and where compensation was an important step) to use dual-laser excitation. Moreover, thanks to recently developed multilaser instruments and novel probes for surface and intracellular markers, JC-1 can be utilized by polychromatic flow cytometry to simultaneously detect, without any compensation between fluorescences, δψm along with other biological parameters, such as apoptosis and the production of reactive oxygen species.
Uncompensated polychromatic analysis of mitochondrial membrane potential using JC-1 and multilaser excitation / DE BIASI, Sara; Gibellini, Lara; Cossarizza, Andrea. - 72(2015), pp. 1-11. [10.1002/0471142956.cy0732s72]
| Data di pubblicazione: | 2015 | |
| Titolo: | Uncompensated polychromatic analysis of mitochondrial membrane potential using JC-1 and multilaser excitation | |
| Autore/i: | DE BIASI, Sara; Gibellini, Lara; Cossarizza, Andrea | |
| Autore/i UNIMORE: | ||
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1002/0471142956.cy0732s72 | |
| Codice identificativo Scopus: | 2-s2.0-84944468646 | |
| Codice identificativo Pubmed: | 25827483 | |
| Serie: | CURRENT PROTOCOLS IN CYTOMETRY | |
| Titolo del libro: | Current Protocols in Cytometry | |
| Nazione editore: | STATI UNITI D'AMERICA | |
| Citazione: | Uncompensated polychromatic analysis of mitochondrial membrane potential using JC-1 and multilaser excitation / DE BIASI, Sara; Gibellini, Lara; Cossarizza, Andrea. - 72(2015), pp. 1-11. [10.1002/0471142956.cy0732s72] | |
| Tipologia | Capitolo/Saggio |
File in questo prodotto:
| File | Descrizione | Tipologia | |
|---|---|---|---|
| 2015_De Biasi_CPC.pdf | Versione dell'editore (versione pubblicata) | Administrator Richiedi una copia | |
| proofs CPC for BP71 JC.pdf | Pre-print dell'autore (bozza pre referaggio) | Open Access Visualizza/Apri |
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