We investigated the presence of ESBL and AmpC-producing Enterobacteriaceae isolated from 200 rectal swabs of healthy swine and 200 samples of ground pork. Phenotypic testing by using the double synergy differential test (DSDT) for ESBL/ AmpC-positive strains was confirmed by PCR and DNA sequence analysis. The localization of beta-lactamase genes was established by conjugation experiments. ESBL and/or AmpC-producing Enterobacteriaceae was found in 52.2% (95/182) of the isolates collected from rectal swabs and 3% (3/100) of isolates obtained from ground pork samples. Polymerase chain reaction and sequencing confirmed the presence of blaTEM-20, blaTEM-34, blaTEM-52, blaCTX-M-1, blaSHV-12, blaTEM-11SHV-12, blaTEM-201SHV-12, blaCMY-2, blaTEM-11 CMY-2, blaACC-1 and blaACC-2. The conjugation assays yielded positive results, denoting a plasmid localization of the genes
EXTENDED-SPECTRUM B-LACTAMASE AND PLASMID-MEDIATED AMPC GENES IN SWINE AND GROUND PORK / Sabia, Carla; Stefani, Sara; Messi, Patrizia; DE NIEDERHAUSERN, Simona; Bondi, Moreno; Condo', Carla; Iseppi, Ramona; Anacarso, Immacolata. - In: JOURNAL OF FOOD SAFETY. - ISSN 0149-6085. - ELETTRONICO. - 37:1(2017), pp. 1-5. [10.1111/jfs.12282]
EXTENDED-SPECTRUM B-LACTAMASE AND PLASMID-MEDIATED AMPC GENES IN SWINE AND GROUND PORK
SABIA, Carla;STEFANI, SARA;MESSI, Patrizia;DE NIEDERHAUSERN, Simona;BONDI, Moreno;CONDO', CARLA;ISEPPI, Ramona;ANACARSO, Immacolata
2017
Abstract
We investigated the presence of ESBL and AmpC-producing Enterobacteriaceae isolated from 200 rectal swabs of healthy swine and 200 samples of ground pork. Phenotypic testing by using the double synergy differential test (DSDT) for ESBL/ AmpC-positive strains was confirmed by PCR and DNA sequence analysis. The localization of beta-lactamase genes was established by conjugation experiments. ESBL and/or AmpC-producing Enterobacteriaceae was found in 52.2% (95/182) of the isolates collected from rectal swabs and 3% (3/100) of isolates obtained from ground pork samples. Polymerase chain reaction and sequencing confirmed the presence of blaTEM-20, blaTEM-34, blaTEM-52, blaCTX-M-1, blaSHV-12, blaTEM-11SHV-12, blaTEM-201SHV-12, blaCMY-2, blaTEM-11 CMY-2, blaACC-1 and blaACC-2. The conjugation assays yielded positive results, denoting a plasmid localization of the genes
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