NF-Y is a heterotrimeric transcription factor, which plays a pioneer role in the transcriptional control of promoters containing the CCAAT-box, among which genes involved in cell cycle regulation, apoptosis and DNA damage response. The knock-down of the sequence-specific subunit NF-YA triggers defects in S-phase progression, which lead to apoptotic cell death. Here, we report that NF-Y has a critical function in DNA replication progression, independent from its transcriptional activity. NF-YA colocalizes with early DNA replication factories, its depletion affects the loading of replisome proteins to DNA, among which Cdc45, and delays the passage from early to middle-late S phase. Molecular combing experiments are consistent with a role for NF-Y in the control of fork progression. Finally, we unambiguously demonstrate a direct non-transcriptional role of NF-Y in the overall efficiency of DNA replication, specifically in the DNA elongation process, using a Xenopus cell-free system. Our findings broaden the activity of NF-Y on a DNA metabolism other than transcription, supporting the existence of specific TFs required for proper and efficient DNA replication.
Direct non transcriptional role of NF-Y in DNA replication / Benatti, Paolo; Belluti, Silvia; Miotto, Benoit; Neusiedler, Julia; Dolfini, Diletta; Drac, Marjorie; Basile, Valentina; Schwob, Ethienne; Mantovani, Roberto; Blow J., Julian; Imbriano, Carol. - In: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH. - ISSN 0167-4889. - ELETTRONICO. - (2016), pp. N/A-N/A. [10.1016/j.bbamcr.2015.12.019]
Data di pubblicazione: | 2016 | |
Data di prima pubblicazione: | 28-dic-2015 | |
Titolo: | Direct non transcriptional role of NF-Y in DNA replication | |
Autore/i: | Benatti, Paolo; Belluti, Silvia; Miotto, Benoit; Neusiedler, Julia; Dolfini, Diletta; Drac, Marjorie; Basile, Valentina; Schwob, Ethienne; Mantovani, Roberto; Blow J., Julian; Imbriano, Carol | |
Autore/i UNIMORE: | ||
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/j.bbamcr.2015.12.019 | |
Rivista: | ||
Pagina iniziale: | N/A | |
Pagina finale: | N/A | |
Codice identificativo ISI: | WOS:000371940500016 | |
Codice identificativo Scopus: | 2-s2.0-84956638822 | |
Codice identificativo Pubmed: | 26732297 | |
Citazione: | Direct non transcriptional role of NF-Y in DNA replication / Benatti, Paolo; Belluti, Silvia; Miotto, Benoit; Neusiedler, Julia; Dolfini, Diletta; Drac, Marjorie; Basile, Valentina; Schwob, Ethienne; Mantovani, Roberto; Blow J., Julian; Imbriano, Carol. - In: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH. - ISSN 0167-4889. - ELETTRONICO. - (2016), pp. N/A-N/A. [10.1016/j.bbamcr.2015.12.019] | |
Tipologia | Articolo su rivista |
File in questo prodotto:
File | Descrizione | Tipologia | |
---|---|---|---|
Benatti P., BBA MCR 2015.pdf | Articolo principale | Versione dell'editore (versione pubblicata) | Open Access Visualizza/Apri |
Pubblicazioni consigliate

I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris