We present a gradient-based optimization method for the estimation of a specimen’s phase function from polychromatic DIC images. The method minimizes the sum of a nonlinear least-squares discrepancy measure and a smooth approximation of the total variation. A new formulation of the gradient and a recent updating rule for the choice of the step size are both exploited to reduce computational time. Numerical simulations on two computer-generated objects show significant improvements, both in efficiency and accuracy, with respect to a more standard choice of the step size.

Phase estimation in Differential-Interference-Contrast (DIC) microscopy / Bautista, Lola; Rebegoldi, Simone; Blanc Féraud, Laure; Prato, Marco; Zanni, Luca; Plata, Arturo. - ELETTRONICO. - (2016), pp. 136-139. ((Intervento presentato al convegno The International Symposium on Biomedical Imaging 2016 tenutosi a Praga nel 13-16 aprile 2016 [10.1109/ISBI.2016.7493229].

Phase estimation in Differential-Interference-Contrast (DIC) microscopy

REBEGOLDI, SIMONE;PRATO, Marco;ZANNI, Luca;
2016

Abstract

We present a gradient-based optimization method for the estimation of a specimen’s phase function from polychromatic DIC images. The method minimizes the sum of a nonlinear least-squares discrepancy measure and a smooth approximation of the total variation. A new formulation of the gradient and a recent updating rule for the choice of the step size are both exploited to reduce computational time. Numerical simulations on two computer-generated objects show significant improvements, both in efficiency and accuracy, with respect to a more standard choice of the step size.
The International Symposium on Biomedical Imaging 2016
Praga
13-16 aprile 2016
136
139
Bautista, Lola; Rebegoldi, Simone; Blanc Féraud, Laure; Prato, Marco; Zanni, Luca; Plata, Arturo
Phase estimation in Differential-Interference-Contrast (DIC) microscopy / Bautista, Lola; Rebegoldi, Simone; Blanc Féraud, Laure; Prato, Marco; Zanni, Luca; Plata, Arturo. - ELETTRONICO. - (2016), pp. 136-139. ((Intervento presentato al convegno The International Symposium on Biomedical Imaging 2016 tenutosi a Praga nel 13-16 aprile 2016 [10.1109/ISBI.2016.7493229].
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/1081786
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 2
  • ???jsp.display-item.citation.isi??? 2
social impact