Biofilm formation is broadly recognized as an important virulence factor in many bacterial species implicated in implant-related opportunistic infections. In spite of a long history of research and many investigative efforts aimed at elucidating their chemical composition, structure, and function, the nature of bacterial biofilms still remains only partly revealed. Over the years, different extracellular polymeric substances (EPS) have been described that contribute functionally and structurally to the organization of biofilms. Recently extracellular DNA (eDNA) has emerged as a quantitatively conspicuous and potentially relevant structural component of microbial biofilms of many microbial species, Staphylococcus aureus and S. epidermidis among them. The present study aims at comparatively investigating the amount of eDNA present in the biofilm of 55 clinical isolates of S. epidermidis from postsurgical and biomaterial-related orthopedic infections. Quantification of eDNA was performed by a non-destructive method directly on bacterial biofilms formed under static conditions on the plastic surface of 96-well plates.

Biofilm extracellular-DNA in 55 Staphylococcus epidermidis clinical isolates from implant infections / Ravaioli, Stefano; Campoccia, Davide; Visai, Livia; Pirini, Valter; Cangini, Ilaria; Corazzari, Tolmino; Maso, Alessandra; Poggio, Claudio; Pegreffi, Francesco; Montanaro, Lucio; Arciola, Carla Renata. - In: INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS. - ISSN 0391-3988. - 34:9(2011), pp. 840-846. [10.5301/ijao.5000057]

Biofilm extracellular-DNA in 55 Staphylococcus epidermidis clinical isolates from implant infections

CORAZZARI, Tolmino;
2011

Abstract

Biofilm formation is broadly recognized as an important virulence factor in many bacterial species implicated in implant-related opportunistic infections. In spite of a long history of research and many investigative efforts aimed at elucidating their chemical composition, structure, and function, the nature of bacterial biofilms still remains only partly revealed. Over the years, different extracellular polymeric substances (EPS) have been described that contribute functionally and structurally to the organization of biofilms. Recently extracellular DNA (eDNA) has emerged as a quantitatively conspicuous and potentially relevant structural component of microbial biofilms of many microbial species, Staphylococcus aureus and S. epidermidis among them. The present study aims at comparatively investigating the amount of eDNA present in the biofilm of 55 clinical isolates of S. epidermidis from postsurgical and biomaterial-related orthopedic infections. Quantification of eDNA was performed by a non-destructive method directly on bacterial biofilms formed under static conditions on the plastic surface of 96-well plates.
2011
34
9
840
846
Biofilm extracellular-DNA in 55 Staphylococcus epidermidis clinical isolates from implant infections / Ravaioli, Stefano; Campoccia, Davide; Visai, Livia; Pirini, Valter; Cangini, Ilaria; Corazzari, Tolmino; Maso, Alessandra; Poggio, Claudio; Pegreffi, Francesco; Montanaro, Lucio; Arciola, Carla Renata. - In: INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS. - ISSN 0391-3988. - 34:9(2011), pp. 840-846. [10.5301/ijao.5000057]
Ravaioli, Stefano; Campoccia, Davide; Visai, Livia; Pirini, Valter; Cangini, Ilaria; Corazzari, Tolmino; Maso, Alessandra; Poggio, Claudio; Pegreffi, ...espandi
File in questo prodotto:
File Dimensione Formato  
IJAO_9_2011_840-846.pdf

Accesso riservato

Tipologia: Versione pubblicata dall'editore
Dimensione 232.17 kB
Formato Adobe PDF
232.17 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1081640
Citazioni
  • ???jsp.display-item.citation.pmc??? 6
  • Scopus 19
  • ???jsp.display-item.citation.isi??? 19
social impact