Neurons are highly polarized cells, and neuron-neuron communication is based on directed transport and release of neurotransmitters, neuropeptides, and neurotrophins. Directed communication may also be attributed to neuron-microglia signaling, since neuronal damage can induce a microglia reaction at specific sites only. However, the mechanism underlying this site-specific microglia reaction is not yet understood. Neuronal CCL21 is a microglia-activating chemokine, which in brain is solely found in endangered neurons and is therefore a candidate for neuron-microglia signaling. Here we present that neuronal CCL21 is sorted into large dense-core vesicles, the secretory granules of the regulated release pathway of neurons. Live-cell imaging studies show preferential sorting of CCL21-containing vesicles into axons, indicating its directed transport. Thus, mouse neurons express and transport a microglia activating factor very similar to signaling molecules used in neuron-neuron communication. These data show for the first time the directed transport of a microglia activating factor in neurons and corroborate the function of neuronal CCL21 in directed neuron-microglia communication.

Expression, transport, and axonal sorting of neuronal CCL21 in large dense-core vesicles / de Jong, Eiko K; Vinet, Jonathan; Stanulovic, Vesna S; Meijer, Michel; Wesseling, Evelyn; Sjollema, Klaas; Boddeke, Hendrikus W. G. M; Biber, Knut. - In: THE FASEB JOURNAL. - ISSN 0892-6638. - 22:(2008), pp. 4136-4145. [10.1096/fj.07-101907]

Expression, transport, and axonal sorting of neuronal CCL21 in large dense-core vesicles

VINET, JONATHAN;
2008

Abstract

Neurons are highly polarized cells, and neuron-neuron communication is based on directed transport and release of neurotransmitters, neuropeptides, and neurotrophins. Directed communication may also be attributed to neuron-microglia signaling, since neuronal damage can induce a microglia reaction at specific sites only. However, the mechanism underlying this site-specific microglia reaction is not yet understood. Neuronal CCL21 is a microglia-activating chemokine, which in brain is solely found in endangered neurons and is therefore a candidate for neuron-microglia signaling. Here we present that neuronal CCL21 is sorted into large dense-core vesicles, the secretory granules of the regulated release pathway of neurons. Live-cell imaging studies show preferential sorting of CCL21-containing vesicles into axons, indicating its directed transport. Thus, mouse neurons express and transport a microglia activating factor very similar to signaling molecules used in neuron-neuron communication. These data show for the first time the directed transport of a microglia activating factor in neurons and corroborate the function of neuronal CCL21 in directed neuron-microglia communication.
22
4136
4145
Expression, transport, and axonal sorting of neuronal CCL21 in large dense-core vesicles / de Jong, Eiko K; Vinet, Jonathan; Stanulovic, Vesna S; Meijer, Michel; Wesseling, Evelyn; Sjollema, Klaas; Boddeke, Hendrikus W. G. M; Biber, Knut. - In: THE FASEB JOURNAL. - ISSN 0892-6638. - 22:(2008), pp. 4136-4145. [10.1096/fj.07-101907]
de Jong, Eiko K; Vinet, Jonathan; Stanulovic, Vesna S; Meijer, Michel; Wesseling, Evelyn; Sjollema, Klaas; Boddeke, Hendrikus W. G. M; Biber, Knut
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/1074598
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