Neurons are highly polarized cells, and neuron-neuron communication is based on directed transport and release of neurotransmitters, neuropeptides, and neurotrophins. Directed communication may also be attributed to neuron-microglia signaling, since neuronal damage can induce a microglia reaction at specific sites only. However, the mechanism underlying this site-specific microglia reaction is not yet understood. Neuronal CCL21 is a microglia-activating chemokine, which in brain is solely found in endangered neurons and is therefore a candidate for neuron-microglia signaling. Here we present that neuronal CCL21 is sorted into large dense-core vesicles, the secretory granules of the regulated release pathway of neurons. Live-cell imaging studies show preferential sorting of CCL21-containing vesicles into axons, indicating its directed transport. Thus, mouse neurons express and transport a microglia activating factor very similar to signaling molecules used in neuron-neuron communication. These data show for the first time the directed transport of a microglia activating factor in neurons and corroborate the function of neuronal CCL21 in directed neuron-microglia communication.
|Data di pubblicazione:||2008|
|Titolo:||Expression, transport, and axonal sorting of neuronal CCL21 in large dense-core vesicles|
|Autore/i:||de Jong, Eiko K; Vinet, Jonathan; Stanulovic, Vesna S; Meijer, Michel; Wesseling, Evelyn; Sjollema, Klaas; Boddeke, Hendrikus W. G. M; Biber, Knut|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1096/fj.07-101907|
|Codice identificativo ISI:||WOS:000261254800013|
|Codice identificativo Scopus:||2-s2.0-57349196489|
|Codice identificativo Pubmed:||18697841|
|Citazione:||Expression, transport, and axonal sorting of neuronal CCL21 in large dense-core vesicles / de Jong, Eiko K; Vinet, Jonathan; Stanulovic, Vesna S; Meijer, Michel; Wesseling, Evelyn; Sjollema, Klaas; Boddeke, Hendrikus W. G. M; Biber, Knut. - In: THE FASEB JOURNAL. - ISSN 0892-6638. - 22(2008), pp. 4136-4145.|
|Tipologia||Articolo su rivista|
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