Aim: Drug targeting to the CNS is challenging due to the presence of blood-brain barrier. We investigated chitosan (Cs) nanoparticles (NPs) as drug transporter system across the blood-brain barrier, based on mAb OX26 modified Cs. Materials & methods: Cs NPs functionalized with PEG, modified and unmodified with OX26 (Cs-PEG-OX26) were prepared and chemico-physically characterized. These NPs were administered (intraperitoneal) in mice to define their ability to reach the brain. Results: Brain uptake of OX26-conjugated NPs is much higher than of unmodified NPs, because: long-circulating abilities (conferred by PEG), interaction between cationic Cs and brain endothelium negative charges and OX26 TfR receptor affinity. Conclusion: Cs-PEG-OX26 NPs are promising drug delivery system to the CNS.
PEG-g-chitosan nanoparticles functionalized with the monoclonal antibody OX26 for brain drug targeting / Monsalve, Yuliana; Tosi, Giovanni; Ruozi, Barbara; Belletti, Daniela; Vilella, Antonietta; Zoli, Michele; Vandelli, Maria Angela; Forni, Flavio; López, Betty L.; Sierra, Ligia. - In: NANOMEDICINE. - ISSN 1743-5889. - STAMPA. - 10:11(2015), pp. 1735-1750. [10.2217/nnm.15.29]
PEG-g-chitosan nanoparticles functionalized with the monoclonal antibody OX26 for brain drug targeting
TOSI, Giovanni;RUOZI, Barbara;BELLETTI, Daniela;VILELLA, ANTONIETTA;ZOLI, Michele;VANDELLI, Maria Angela;FORNI, Flavio;
2015
Abstract
Aim: Drug targeting to the CNS is challenging due to the presence of blood-brain barrier. We investigated chitosan (Cs) nanoparticles (NPs) as drug transporter system across the blood-brain barrier, based on mAb OX26 modified Cs. Materials & methods: Cs NPs functionalized with PEG, modified and unmodified with OX26 (Cs-PEG-OX26) were prepared and chemico-physically characterized. These NPs were administered (intraperitoneal) in mice to define their ability to reach the brain. Results: Brain uptake of OX26-conjugated NPs is much higher than of unmodified NPs, because: long-circulating abilities (conferred by PEG), interaction between cationic Cs and brain endothelium negative charges and OX26 TfR receptor affinity. Conclusion: Cs-PEG-OX26 NPs are promising drug delivery system to the CNS.File | Dimensione | Formato | |
---|---|---|---|
Monsalve et al., Nanomedicine 2015.pdf
Accesso riservato
Tipologia:
Versione pubblicata dall'editore
Dimensione
4.47 MB
Formato
Adobe PDF
|
4.47 MB | Adobe PDF | Visualizza/Apri Richiedi una copia |
Manuscript_rev_accepted.pdf
Open access
Tipologia:
Versione dell'autore revisionata e accettata per la pubblicazione
Dimensione
510.41 kB
Formato
Adobe PDF
|
510.41 kB | Adobe PDF | Visualizza/Apri |
Pubblicazioni consigliate
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris