New octapeptides able to reduce the growth of platinum-resistant cells by inhibiting the enzyme human thymidylate synthase (hTS), cannot cross the cell membrane alone and require an appropriate delivery system. In the aim to transport hTS inhibiting LR octapeptide (LR-op) into the cells, Solid Lipid Nanoparticles (SLNs) were developed and evaluated in vitro. The optimized SLNs were formulated in the absence and presence of squalene (7S and 7Sq) both in the LR-op loaded and unloaded form. All the SLNs produced had dimensions below 150 nm, negative Zpotential and a good stability both in suspension and after freeze-drying. Only the sample obtained in the absence of squalene showed to stably incorporate the LR-op promoting its cell internalization, as demonstrated by in vitro studies on C13* ovarian carcinoma cell line.

Nanoencapsulation of an hTS inhibitor octapeptide against ovarian cancer in solid lipid matrix / Sacchetti, Francesca; Marraccini, Chiara; Cannazza, Giuseppe; Iannuccelli, Valentina; Hanuskova, Miriam; Maretti, Eleonora; Costi, Maria Paola; Leo, Eliana Grazia. - (2015). (Intervento presentato al convegno 42nd CRS Annual Meeting & Exposition tenutosi a Edinburgh, Scotland nel July 26-29, 2015).

Nanoencapsulation of an hTS inhibitor octapeptide against ovarian cancer in solid lipid matrix

SACCHETTI, FRANCESCA;MARRACCINI, CHIARA;CANNAZZA, Giuseppe;IANNUCCELLI, Valentina;HANUSKOVA, Miriam;MARETTI, ELEONORA;COSTI, Maria Paola;LEO, Eliana Grazia
2015

Abstract

New octapeptides able to reduce the growth of platinum-resistant cells by inhibiting the enzyme human thymidylate synthase (hTS), cannot cross the cell membrane alone and require an appropriate delivery system. In the aim to transport hTS inhibiting LR octapeptide (LR-op) into the cells, Solid Lipid Nanoparticles (SLNs) were developed and evaluated in vitro. The optimized SLNs were formulated in the absence and presence of squalene (7S and 7Sq) both in the LR-op loaded and unloaded form. All the SLNs produced had dimensions below 150 nm, negative Zpotential and a good stability both in suspension and after freeze-drying. Only the sample obtained in the absence of squalene showed to stably incorporate the LR-op promoting its cell internalization, as demonstrated by in vitro studies on C13* ovarian carcinoma cell line.
2015
42nd CRS Annual Meeting & Exposition
Edinburgh, Scotland
July 26-29, 2015
Sacchetti, Francesca; Marraccini, Chiara; Cannazza, Giuseppe; Iannuccelli, Valentina; Hanuskova, Miriam; Maretti, Eleonora; Costi, Maria Paola; Leo, E...espandi
Nanoencapsulation of an hTS inhibitor octapeptide against ovarian cancer in solid lipid matrix / Sacchetti, Francesca; Marraccini, Chiara; Cannazza, Giuseppe; Iannuccelli, Valentina; Hanuskova, Miriam; Maretti, Eleonora; Costi, Maria Paola; Leo, Eliana Grazia. - (2015). (Intervento presentato al convegno 42nd CRS Annual Meeting & Exposition tenutosi a Edinburgh, Scotland nel July 26-29, 2015).
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1071041
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact