Human keratinocytes isolated from a skin biopsy and cultured in vitro on a feeder-layer of irradiated fibroblasts reconstitute a stratified squamous epithelium suitable for grafting onto patients suffering from large burn wounds. Since conditioned medium from 3T3-J2 cells can partially substitute for the intact feeder-layer, we studied the possible involvement of insulin-like growth factors acting in a paracrine fashion. IGFs were measured (after Sephadex G-50 gel-chromatography in acid conditions) in media conditioned by a feeder-layer of lethally irradiated 3T3-J2 fibroblasts on which keratinocytes were grown. Immunoreactive (IR) IGF-I, IGF-II, and IGF binding activity were present in the medium conditioned by the feeder-layer. The medium conditioned by keratinocytes showed nearly undetectable amounts of IR IGF-I and IGF-II, suggesting that keratinocytes are unable to synthesize IGFs peptides. Recombinant IGF-I and IGF-II, and conditioned medium from 3T3-J2 cells, caused a dose-dependent increase of 3H-thymydine incorporation in cultured keratinocytes. The stimulatory effect of IGF and of 3T3-J2 conditioned medium was inhibited by the MoAb Sm 1.2, which recognizes both IGF-I and IGF-II but not insulin, and by the MoAb alpha IR-3, which is a specific antagonist of type-I IGF receptor. Fetal mouse-derived 3T3-J2 cells and adult human skin fibroblasts were equally able to sustain keratinocyte growth and in both cases addition of Sm 1.2 MoAb causes a 50% decrease in the keratinocyte number. When the non-IGF-producing BALB/c 3T3 cells were used as a feeder-layer, the keratinocytes number was similar to that observed with 3T3-J2 and with human fibroblasts plus the Sm 1.2 MoAb. IGF-I and IGF-II restored the BALB/c 3T3 growth promoting activity to the level of 3T3-J2 and of normal human fibroblasts. Our results suggest that fetal mouse 3T3-J2 and human fibroblasts synthesize IGF peptides, while keratinocytes do not. Fibroblast-derived IGFs stimulate keratinocyte growth in a paracrine fashion, suggesting their role in the regulation of keratinocyte proliferation in skin growth and in wound healing.
In vitro paracrine regulation of human keratinocyte growth by fibroblast-derived insulin-like growth factors / Barreca, A; DE LUCA, Michele; Del Monte, P; Bondanza, S; Damonte, G; Cariola, G; Di Marco, E; Giordano, G; Cancedda, R; Minuto, F.. - In: JOURNAL OF CELLULAR PHYSIOLOGY. - ISSN 0021-9541. - STAMPA. - 151(1992), pp. 262-268. [10.1002/jcp.1041510207]
Data di pubblicazione: | 1992 | |
Titolo: | In vitro paracrine regulation of human keratinocyte growth by fibroblast-derived insulin-like growth factors | |
Autore/i: | Barreca, A; DE LUCA, Michele; Del Monte, P; Bondanza, S; Damonte, G; Cariola, G; Di Marco, E; Giordano, G; Cancedda, R; Minuto, F. | |
Autore/i UNIMORE: | ||
Digital Object Identifier (DOI): | http://dx.doi.org/10.1002/jcp.1041510207 | |
Rivista: | ||
Volume: | 151 | |
Pagina iniziale: | 262 | |
Pagina finale: | 268 | |
Codice identificativo ISI: | WOS:A1992HR76000006 | |
Codice identificativo Scopus: | 2-s2.0-0026605632 | |
Codice identificativo Pubmed: | 1374070 | |
Citazione: | In vitro paracrine regulation of human keratinocyte growth by fibroblast-derived insulin-like growth factors / Barreca, A; DE LUCA, Michele; Del Monte, P; Bondanza, S; Damonte, G; Cariola, G; Di Marco, E; Giordano, G; Cancedda, R; Minuto, F.. - In: JOURNAL OF CELLULAR PHYSIOLOGY. - ISSN 0021-9541. - STAMPA. - 151(1992), pp. 262-268. [10.1002/jcp.1041510207] | |
Tipologia | Articolo su rivista |
File in questo prodotto:
Pubblicazioni consigliate

I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris