In human keratinocytes, mediated transport of Cl- was found to occur mainly by two mechanisms: an anion exchange and an electrically conductive pathway. The contribution of the anion exchange, which accounted for about 50% of overall Cl- efflux, was assessed either by its sensitivity to inhibition by 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS), and by means of Cl- substitution experiments. The anion exchange exhibited a saturation behaviour over the range 10-135 mM Cl-; Cl- was more efficient than HCO3-, Br- and NO3- in increasing Cl- efflux rate, whereas SO4(2-) and I- inhibited Cl- efflux. The electrically conductive Cl- pathway, which accounted for about 40% of total Cl- efflux, was inhibited by the Cl- channel blocker 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB) and was at least partially sensitive to variation of the plasma membrane potential. The Cl- channel was insensitive to elevation in the intracellular concentration of either cyclic AMP and calcium ions. Indomethacin, an inhibitor of the cyclooxygenase, failed to reduce Cl- efflux, whereas nordihydroguaiaretic acid (NDGA), an inhibitor of the lipoxygenase, induced 50% inhibition of Cl- efflux. These results support the conclusion that endogenous production of lipoxygenase-derived arachidonic acid metabolite(s) might be responsible for high basal Cl- permeability in human keratinocytes.
Characterization of chloride transport pathways in cultured human keratinocytes / Mastrocola, T; DE LUCA, Michele; Rugolo, M.. - In: BIOCHIMICA ET BIOPHYSICA ACTA. - ISSN 0006-3002. - STAMPA. - 1097(1991), pp. 275-282.
Data di pubblicazione: | 1991 |
Titolo: | Characterization of chloride transport pathways in cultured human keratinocytes |
Autore/i: | Mastrocola, T; DE LUCA, Michele; Rugolo, M. |
Autore/i UNIMORE: | |
Rivista: | |
Volume: | 1097 |
Pagina iniziale: | 275 |
Pagina finale: | 282 |
Codice identificativo ISI: | WOS:A1991GT80800004 |
Codice identificativo Scopus: | 2-s2.0-0025939623 |
Codice identificativo Pubmed: | 1742332 |
Citazione: | Characterization of chloride transport pathways in cultured human keratinocytes / Mastrocola, T; DE LUCA, Michele; Rugolo, M.. - In: BIOCHIMICA ET BIOPHYSICA ACTA. - ISSN 0006-3002. - STAMPA. - 1097(1991), pp. 275-282. |
Tipologia | Articolo su rivista |
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