Protein folding is still a major area of active research. Despite significant progress in understanding the underlying principles, we still cannot efficiently predict the folding mechanism for even a moderately sized protein. Proteins are generally thought to fold by diffusion over a three-dimensional energy landscape. Traditional bulk methods have proven to be very powerful in the study of the folding process but they often suffer from inherent ensemble averaging. Single molecule techniques open up new vistas for studying protein folding, allowing direct analysis of the distribution of events that characterize the heterogeneous folding process. Recently it has become possible to directly manipulate individual proteins using optical tweezers. Here we illustrate the experimental strategy and how this approach has provided a fresh perspective on the protein folding problem.

Exploring Folding Pathways of Single Proteins Using Mechanical Manipulation / Heidarsson, Petur O.; Cecconi, Ciro. - 1456:(2012), pp. 201-206. (Intervento presentato al convegno International Congress in Theory and Applications in Computational Chemistry: The First Decade of the Second Millennium, TACC 2012 tenutosi a University of Pavia, ita nel SEP 02-07, 2012) [10.1063/1.4730660].

Exploring Folding Pathways of Single Proteins Using Mechanical Manipulation

CECCONI, CIRO
2012

Abstract

Protein folding is still a major area of active research. Despite significant progress in understanding the underlying principles, we still cannot efficiently predict the folding mechanism for even a moderately sized protein. Proteins are generally thought to fold by diffusion over a three-dimensional energy landscape. Traditional bulk methods have proven to be very powerful in the study of the folding process but they often suffer from inherent ensemble averaging. Single molecule techniques open up new vistas for studying protein folding, allowing direct analysis of the distribution of events that characterize the heterogeneous folding process. Recently it has become possible to directly manipulate individual proteins using optical tweezers. Here we illustrate the experimental strategy and how this approach has provided a fresh perspective on the protein folding problem.
2012
International Congress in Theory and Applications in Computational Chemistry: The First Decade of the Second Millennium, TACC 2012
University of Pavia, ita
SEP 02-07, 2012
1456
201
206
Heidarsson, Petur O.; Cecconi, Ciro
Exploring Folding Pathways of Single Proteins Using Mechanical Manipulation / Heidarsson, Petur O.; Cecconi, Ciro. - 1456:(2012), pp. 201-206. (Intervento presentato al convegno International Congress in Theory and Applications in Computational Chemistry: The First Decade of the Second Millennium, TACC 2012 tenutosi a University of Pavia, ita nel SEP 02-07, 2012) [10.1063/1.4730660].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1070002
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