Nannofossil data from ODP Site 711 (equatorial Indian Ocean) yield a set of consistent, reliable biohorizons that form the basis of a revised calcareous nannofossil biostratigraphy for the low-latitude Eocene-Oligocene. We discuss 31 biohorizons occurring over an 11 myr time interval which we correlate to previous magnetostratigraphic data. Calcareous nannofossils from the middle Eocene through the lower Oligocene of the studied section are characterized bymoderately well preserved assemblages consisting largely of lowlatitude and cosmopolitan species. A significant nannofossil dissolution interval is evidenced at the middle Eocene Climatic Optimum (MECO). We document a significant increase in late Eocene nannoplankton exhibiting a eutrophic preference. Analysis of the assemblage suggests important changes in the equatorial oceanic regime just before the onset of the Eocene-Oligocene transition (EOT), that foreshadow the more dramatic climatic shift of the early Oligocene.