Human peroxidasin 1 (hsPxd01) is a multidomain heme peroxidase that uses bromide as a cofactor for the formation of sulfilimine crosslinks. The latter confer critical structural reinforcement to collagen IV scaffolds. Here hsPxd01 and various truncated variants lacking non-enzymatic domains were recombinantly expressed in HEK cell lines. The N-glycosylation site occupancy and disulfide pattern, the oligomeric structure and unfolding pathway are reported. The homotrimeric ironprotein contains a covalently-bound ferric high-spin heme per subunit with a standard reduction potential of the Fe(III)/Fe(II) couple of -233 mV at pH 7.0. Despite sequence homology at the active site and biophysical properties similar to human peroxidases, the catalytic efficiency of bromide oxidation (kcat/KM) of full-length hsPxd01 is rather low but increased upon truncation. This is discussed with respect to its structure and proposed biosynthetic function in collagen IV crosslinking.
Multidomain human peroxidasin 1 is a highly glycosylated and stable homotrimeric high-spin ferric peroxidase / Soudi, Monika; Delporte, Cedric; Paumann Page, Martina; Pirker, Katharina F.; Bellei, Marzia; Edenhofer, Eva; Stadlmayr, Gerhard; Battistuzzi, Gianantonio; Furtmüller, Paul G.; Van Antwerpen, Pierre; Obinger, Christian; Boudjeltia, Karim Zouaoui. - In: JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 1083-351X. - STAMPA. - 290:17(2015), pp. 10876-10890.
Data di pubblicazione: | 2015 |
Data di prima pubblicazione: | 24-feb-2015 |
Titolo: | Multidomain human peroxidasin 1 is a highly glycosylated and stable homotrimeric high-spin ferric peroxidase |
Autore/i: | Soudi, Monika; Delporte, Cedric; Paumann Page, Martina; Pirker, Katharina F.; Bellei, Marzia; Edenhofer, Eva; Stadlmayr, Gerhard; Battistuzzi, Gianantonio; Furtmüller, Paul G.; Van Antwerpen, Pierre; Obinger, Christian; Boudjeltia, Karim Zouaoui |
Autore/i UNIMORE: | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1074/jbc.M114.632273 |
Rivista: | |
Volume: | 290 |
Fascicolo: | 17 |
Pagina iniziale: | 10876 |
Pagina finale: | 10890 |
Codice identificativo ISI: | WOS:000353404500027 |
Codice identificativo Scopus: | 2-s2.0-84928403603 |
Codice identificativo Pubmed: | 25713063 |
Citazione: | Multidomain human peroxidasin 1 is a highly glycosylated and stable homotrimeric high-spin ferric peroxidase / Soudi, Monika; Delporte, Cedric; Paumann Page, Martina; Pirker, Katharina F.; Bellei, Marzia; Edenhofer, Eva; Stadlmayr, Gerhard; Battistuzzi, Gianantonio; Furtmüller, Paul G.; Van Antwerpen, Pierre; Obinger, Christian; Boudjeltia, Karim Zouaoui. - In: JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 1083-351X. - STAMPA. - 290:17(2015), pp. 10876-10890. |
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