The heterotrimeric transcription factor NF-Y, composed by NF-YA, NF-YB and NF-YC subunits, is a key transcriptional regulator of cell cycle progression. Using data generated by ENCODE, we identified a striking overlap between loci bound by NF-Y-and ORC2, hinting at a possible role of NF-Y in DNA replication. NF-YA knock-down leads to replication defects and the activation an intra-S checkpoint. We investigated the role of NF-Y in DNA replication by using the Xenopus cell-free system. NF-Y subunits were found to be recruited to chromatin during DNA replication. Both immunodepletion of NF-YA or NF-YB and overexpression of a dominant-negative NF-YA mutant lead to a clear decrease in DNA synthesis. In mammalian cells, NF-Y colocalizes and directly interacts with DNA replication proteins. Nascent strand abundance assay in NF-YA inactivated cells corroborates that NF-Y participates to the DNA replication process. Our data highlight that, in addition to its transcriptional activity in controlling cell proliferation, NF-Y plays a key role in the non transcriptional control of DNA replication.
|Data di pubblicazione:||2013|
|Titolo:||A non transcriptional role for NF-Y in DNA replication|
|Autore/i:||Benatti, Paolo; Belluti, Silvia; Neusiedler, Julia; Basile, Valentina; Blow, J. Julian; Imbriano, Carol|
|Data del convegno:||5-7/06/2013|
|Luogo del convegno:||Pavia|
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