HR-MAS NMR spectroscopy was employed to monitor the metabolic profiles of Modena BioBank breast samples over 1 year of freezing at -80 C. The study includes 22 adult female patients living in Modena and its hinterland, who underwent total mastectomy or quadrantectomy in 2011–2012. Variations occur, especially affecting phosphocholine (PC) and choline. This is not a trivial finding, since many studies base the distinction between neoplastic and healthy tissues or the assessment of tumor grade on the analysis of choline metabolites. Despite the changes observed, we established that the diagnostic power of the HR-MAS NMR spectra of frozen samples is preserved, at least as far as the distinction between neoplastic and healthy samples is concerned. Lactate (Lac), PC, phosphoethanolamine (PE), taurine (Tau), myo-inositol (Myo) and glucose (Glc) are biomarkers that can be used to distinguish healthy from neoplastic tissues, whereas some metabolite ratios, such as Lac ? PE ? Tau/Glc ? Myo, seem to have even higher discrimination potential.

Assessment of freezing effects and diagnostic potential of BioBank healthy and neoplastic breast tissues through HR-MAS NMR spectroscopy / Righi, Valeria; Schenetti, Luisa; Maiorana, Antonino; Libertini, Emanuela; Bettelli, Stefania Raffaella; Reggiani Bonetti, Luca; Mucci, Adele. - In: METABOLOMICS. - ISSN 1573-3882. - STAMPA. - 11:2(2015), pp. 487-498. [10.1007/s11306-014-0709-z]

Assessment of freezing effects and diagnostic potential of BioBank healthy and neoplastic breast tissues through HR-MAS NMR spectroscopy

SCHENETTI, Luisa;MAIORANA, Antonino;LIBERTINI, Emanuela;BETTELLI, Stefania Raffaella;REGGIANI BONETTI, Luca;MUCCI, Adele
2015

Abstract

HR-MAS NMR spectroscopy was employed to monitor the metabolic profiles of Modena BioBank breast samples over 1 year of freezing at -80 C. The study includes 22 adult female patients living in Modena and its hinterland, who underwent total mastectomy or quadrantectomy in 2011–2012. Variations occur, especially affecting phosphocholine (PC) and choline. This is not a trivial finding, since many studies base the distinction between neoplastic and healthy tissues or the assessment of tumor grade on the analysis of choline metabolites. Despite the changes observed, we established that the diagnostic power of the HR-MAS NMR spectra of frozen samples is preserved, at least as far as the distinction between neoplastic and healthy samples is concerned. Lactate (Lac), PC, phosphoethanolamine (PE), taurine (Tau), myo-inositol (Myo) and glucose (Glc) are biomarkers that can be used to distinguish healthy from neoplastic tissues, whereas some metabolite ratios, such as Lac ? PE ? Tau/Glc ? Myo, seem to have even higher discrimination potential.
27-lug-2014
11
2
487
498
Assessment of freezing effects and diagnostic potential of BioBank healthy and neoplastic breast tissues through HR-MAS NMR spectroscopy / Righi, Valeria; Schenetti, Luisa; Maiorana, Antonino; Libertini, Emanuela; Bettelli, Stefania Raffaella; Reggiani Bonetti, Luca; Mucci, Adele. - In: METABOLOMICS. - ISSN 1573-3882. - STAMPA. - 11:2(2015), pp. 487-498. [10.1007/s11306-014-0709-z]
Righi, Valeria; Schenetti, Luisa; Maiorana, Antonino; Libertini, Emanuela; Bettelli, Stefania Raffaella; Reggiani Bonetti, Luca; Mucci, Adele
File in questo prodotto:
File Dimensione Formato  
CorrectedProof_Part 1.pdf

accesso aperto

Descrizione: Prima parte
Tipologia: Post-print dell'autore (bozza post referaggio)
Dimensione 5.24 MB
Formato Adobe PDF
5.24 MB Adobe PDF Visualizza/Apri
CorrectedProof_Part 2.pdf

accesso aperto

Descrizione: Seconda parte
Tipologia: Post-print dell'autore (bozza post referaggio)
Dimensione 5.32 MB
Formato Adobe PDF
5.32 MB Adobe PDF Visualizza/Apri
Metabolomics2015.pdf

non disponibili

Tipologia: Versione dell'editore (versione pubblicata)
Dimensione 3.29 MB
Formato Adobe PDF
3.29 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/1065373
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 2
  • ???jsp.display-item.citation.isi??? 2
social impact