The dissemination of carbapenemase-producing Klebsiella pneumoniae (KPC) strains is an increasing problem worldwide. KPC ß-lactamases are Ambler class A enzymes mostly plasmid-encoded; their global spread represents a threat to clinical patients care and public health. Between September-December 2012, have been isolated 60 Klebsiella pneumoniae strains that showed a decrease of susceptibility to carbapenems, collected from patients at the Provincial Laboratory of Clinical Microbiology ‘S. Agostino-Estense’ Hospital (Modena, Italy). Species identification and antimicrobial susceptibility testing were carried out using the Vitek 2 and studied for carbapenemases production using phenotypic confirmatory tests. The presence of carbapenemase genes were confirmed by PCR and sequencing. The localization of beta-lactamase genes was established by conjugation experiments. We reported the emergence of KPC-2-producing Klebsiella pneumoniae isolates PCR and sequencing experiments revealed the presence of blaKPC-2 gene in 56/60 isolates. The KPC-encoding plasmid was analysed by transconjugation experiments and DNA sequencing. The findings indicate the need to supplement surveillance systems based on susceptibility data with the surveillance of resistance mechanisms.
|Data di pubblicazione:||2014|
|Titolo:||Identification and Characterization of KPC-Producing Klebsiella pneumoniae in Clinical Isolates|
|Autori:||Marchi, Sara; Giovanelli, Ilaria; Gargiulo, Raffaele; Nozzi, Daniela; Barozzi, Agostino; Sarti, Mario; Iseppi, Ramona; Anacarso, Immacolata; Sabia, Carla; Sciences, * 1Department of Life; Emilia, University of Modena and Reggio; Modena, ; Microbiology, Italy 2Provincial Laboratory of Clinical; Hospital, S. Agostino-Estense; Modena, ; Carlasabia@unimoreit, Italy *Corresponding Author: firstname.lastname@example.org|
|Digital Object Identifier (DOI):||10.13189/ujmr.2014.020301|
|Appare nelle tipologie:||Articolo su rivista|
File in questo prodotto:
I documenti presenti in Iris Unimore sono rilasciati con licenza Creative Commons Attribuzione - Non commerciale - Non opere derivate 3.0 Italia, salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris