Transplantation of retina pigment epithelium (RPE) has great potentials for the cure of several retinal degenerative diseases such as age-related macular degeneration (AMD). The source of cells for this type of therapy is still under evaluation even if clinical trials with RPE derived from human embryonic stem cells have recently started. We evaluated the ciliary body as a source of cells to differentiate RPE in vitro. The ciliary body is an accessible tissue that can be easily obtained from post-mortem donors. Retinal neurospheres can be generated from ciliary body cells after dissociation and growth in clonal conditions. We deeply characterized differentiation of RPE cells from retinal neurospheres derived from murine eyes. We will also discuss the differentiation protocol developed for the human tissue.
Differentiation of retinal pigment epithelium from retinal neurospheres of the adult ciliary body / Bertolotti, Evelina; Aruta, CLAUDIA GAETANA; Giordano, Francesca; Raposo, Graça; Marigo, Valeria. - In: PIGMENT CELL & MELANOMA RESEARCH. - ISSN 1755-148X. - STAMPA. - 26:Suppl. 5(2013), pp. E16-E16. (Intervento presentato al convegno 18th Meeting of the European Society for Pigment Cell Research tenutosi a Lisbona, PT nel 9-12 settembre 2013).
Differentiation of retinal pigment epithelium from retinal neurospheres of the adult ciliary body
BERTOLOTTI, EVELINA;ARUTA, CLAUDIA GAETANA;MARIGO, Valeria
2013
Abstract
Transplantation of retina pigment epithelium (RPE) has great potentials for the cure of several retinal degenerative diseases such as age-related macular degeneration (AMD). The source of cells for this type of therapy is still under evaluation even if clinical trials with RPE derived from human embryonic stem cells have recently started. We evaluated the ciliary body as a source of cells to differentiate RPE in vitro. The ciliary body is an accessible tissue that can be easily obtained from post-mortem donors. Retinal neurospheres can be generated from ciliary body cells after dissociation and growth in clonal conditions. We deeply characterized differentiation of RPE cells from retinal neurospheres derived from murine eyes. We will also discuss the differentiation protocol developed for the human tissue.
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