The study was aimed at developing a new analytical method for the metabolite fingerprinting of bioactive compounds in Humulus lupulus L. (hop), together with a simple extraction procedure. Different extraction techniques, including maceration, heat reflux extraction (HRE), ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE), were compared in order to obtain a high yield of the target analytes. Dynamic maceration for 30min with MeOH-HCOOH (99:1, v/v) as the extraction solvent provided the best result in terms of recovery of secondary metabolites. The analysis of hop constituents, including prenylflavonoids and prenylphloroglucinols (bitter acids), was carried out by means of HPLC-UV/DAD, HPLC-ESI-MS and MS(2), using an ion trap mass analyzer. An Ascentis Express C18 column (150mm×3.0mm I.D., 2.7μm) was used for the HPLC analysis, with a mobile phase composed of 0.25% formic acid in both water and acetonitrile, under gradient elution. The method validation was performed to show compliance with ICH guidelines. The validated technique was successfully applied to the phytochemical analysis of ten commercial cultivars and twenty-three wild Italian hop genotypes, thus demonstrating to be a reliable and useful tool for the comprehensive multi-component analysis of hop secondary metabolites.

Development of a new high-performance liquid chromatography method with diode array and electrospray ionization-mass spectrometry detection for the metabolite fingerprinting of bioactive compounds in Humulus lupulus L / Prencipe, FRANCESCO PIO; Brighenti, Virginia; Rodolfi, Margherita; Mongelli, Andrea; Dall'Asta, Chiara; Ganino, Tommaso; Bruni, Renato; Pellati, Federica. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 0021-9673. - ELETTRONICO. - 1349:(2014), pp. 50-59. [10.1016/j.chroma.2014.04.097]

Development of a new high-performance liquid chromatography method with diode array and electrospray ionization-mass spectrometry detection for the metabolite fingerprinting of bioactive compounds in Humulus lupulus L

PRENCIPE, FRANCESCO PIO;BRIGHENTI, VIRGINIA;PELLATI, Federica
2014

Abstract

The study was aimed at developing a new analytical method for the metabolite fingerprinting of bioactive compounds in Humulus lupulus L. (hop), together with a simple extraction procedure. Different extraction techniques, including maceration, heat reflux extraction (HRE), ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE), were compared in order to obtain a high yield of the target analytes. Dynamic maceration for 30min with MeOH-HCOOH (99:1, v/v) as the extraction solvent provided the best result in terms of recovery of secondary metabolites. The analysis of hop constituents, including prenylflavonoids and prenylphloroglucinols (bitter acids), was carried out by means of HPLC-UV/DAD, HPLC-ESI-MS and MS(2), using an ion trap mass analyzer. An Ascentis Express C18 column (150mm×3.0mm I.D., 2.7μm) was used for the HPLC analysis, with a mobile phase composed of 0.25% formic acid in both water and acetonitrile, under gradient elution. The method validation was performed to show compliance with ICH guidelines. The validated technique was successfully applied to the phytochemical analysis of ten commercial cultivars and twenty-three wild Italian hop genotypes, thus demonstrating to be a reliable and useful tool for the comprehensive multi-component analysis of hop secondary metabolites.
1349
50
59
Development of a new high-performance liquid chromatography method with diode array and electrospray ionization-mass spectrometry detection for the metabolite fingerprinting of bioactive compounds in Humulus lupulus L / Prencipe, FRANCESCO PIO; Brighenti, Virginia; Rodolfi, Margherita; Mongelli, Andrea; Dall'Asta, Chiara; Ganino, Tommaso; Bruni, Renato; Pellati, Federica. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 0021-9673. - ELETTRONICO. - 1349:(2014), pp. 50-59. [10.1016/j.chroma.2014.04.097]
Prencipe, FRANCESCO PIO; Brighenti, Virginia; Rodolfi, Margherita; Mongelli, Andrea; Dall'Asta, Chiara; Ganino, Tommaso; Bruni, Renato; Pellati, Federica
File in questo prodotto:
File Dimensione Formato  
Humulus lupulus JCA.pdf

non disponibili

Tipologia: Post-print dell'autore (bozza post referaggio)
Dimensione 854.01 kB
Formato Adobe PDF
854.01 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/1062575
Citazioni
  • ???jsp.display-item.citation.pmc??? 11
  • Scopus 45
  • ???jsp.display-item.citation.isi??? 43
social impact