This paper reports a RP-HPLC method for the determination in topics of azelaic acid, a keratolytic and anti-comedogenic agent widely used in the treatment of all types of acne. A derivatization step was needed prior to chromatographic analysis because the analyte is lacking in chromophore. A sample clean-up procedure by solid-phase extraction was also developed to analyse azelaic acid in complex matrices, such as pharmaceutical and cosmetic formulations.
DETERMINATION OF AZELAIC ACID IN PHARMACEUTICALS AND COSMETICS BY RP-HPLC AFTER PRECOLUMN DERIVATIZATION / Ferioli, Valeria; Rustichelli, Cecilia; Vezzalini, Francesca; Gamberini, Gianfranco. - In: IL FARMACO. - ISSN 0014-827X. - STAMPA. - 49:(1994), pp. 421-425.