Codling moth populations from Emilia-Romagna orchards with difficulties in pest control have been studied combining biochemical and molecular tests, bioassays and small scale field trials. Biochemical assays aimed to evaluate EST, GST and MFO activities, put in evidence that the field populations examined have mainly developed a MFO detoxifying response, in some cases supported by EST and/or GST contributes. A direct sequencing strategy of the AChE-1 gene showed that none of the analyzed strains possessed target-site mutations within AChE-1 sequence. Field trials and bioassays revealed that increased MFO was frequently associated to a reduction of IGR sensibility (tebufenozide and diflubenzuron) and, in less extend, to chlorpyrifos.
Biochemical, molecular and field monitoring of insecticide resistance in codling moth populations collected in Emilia Romagna (Italy) orchards / M., Rivi; Cassanelli, Stefano; A., Butturini; E., Pasqualini; M., Boselli; S., Civolani; S., Caruso; Manicardi, Gian Carlo. - STAMPA. - 74:(2012), pp. 83-88. (Intervento presentato al convegno Integrated Protection of Fruit Crops Subgroups „Pome fruit arthropods“ and „Stone fruits“ tenutosi a Vico Garganico nel 12-17 September 2010).
Biochemical, molecular and field monitoring of insecticide resistance in codling moth populations collected in Emilia Romagna (Italy) orchards
CASSANELLI, Stefano;MANICARDI, Gian Carlo
2012
Abstract
Codling moth populations from Emilia-Romagna orchards with difficulties in pest control have been studied combining biochemical and molecular tests, bioassays and small scale field trials. Biochemical assays aimed to evaluate EST, GST and MFO activities, put in evidence that the field populations examined have mainly developed a MFO detoxifying response, in some cases supported by EST and/or GST contributes. A direct sequencing strategy of the AChE-1 gene showed that none of the analyzed strains possessed target-site mutations within AChE-1 sequence. Field trials and bioassays revealed that increased MFO was frequently associated to a reduction of IGR sensibility (tebufenozide and diflubenzuron) and, in less extend, to chlorpyrifos.
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