Background: Autosomal dominant polycystic kidney disease is a condition mainly character- ized by the progressive development and enlar- gement of cysts in each kidney. In this process a high rate of proliferation and apoptosis of tubu- lar cells has been documented and interpreted as a futile attempt of tissue repair. In considera- tion of the role of stem cells in reparative proc- esses we investigated the presence and local- ization of CD133 + CD24+ renal progenitors in renal ADPKD tissue and cells. Methods: Two normal kidneys and two ADPKD kidneys were examined. CD133 and CD24 expression was in- vestigated by confocal microscopy and immu- noblotting. Furthermore cystic isolated cells and cultured immortalized cells were characterized. Results: CD133 and CD24 have the same local- ization in ADPKD tissues and in normal kidneys: expression is restricted to a subset of epithelial cells (PEC) of Bowman’s capsule and to tubular cells in a focal and segmental pattern. Further- more, in ADPKD tissue, cysts diffusely express CD133 and CD24. According to a quantitative analysis in ADPKD tissue CD133 + CD24 + cells are statistically more expressed in tubules (p < 0.001) and less expressed in the Bowman’s capsule (p = 0.0016) compared to the same lo- calizations in control tissue. Conclusions: CD133 and CD24 antigens, typically expressed by renal epithelial progenitors, are more expressed in ADPKD tubules and highly expressed in ADPKD cysts. Whether CD133 and CD24 expression would signify renal progenitor recruitment or alternatively an expression pattern of the dedif- ferentiation of ADPKD cells remains unclear.

CD133 and CD24 expression in renal tissue of patients affected by autosomal dominant polcystic kidney disease / Lodi, Daniele; Ligabue, Giulia; Cavazzini, Fabrizio; Lupo, Valentina; Cappelli, Gianni; Magistroni, Riccardo. - In: STEM CELL DISCOVERY. - ISSN 2161-6760. - ELETTRONICO. - 3:(2013), pp. 211-217. [10.4236/scd.2013.34026]

CD133 and CD24 expression in renal tissue of patients affected by autosomal dominant polcystic kidney disease

LODI, DANIELE;LIGABUE, Giulia;CAVAZZINI, FABRIZIO;LUPO, Valentina;CAPPELLI, Gianni;MAGISTRONI, Riccardo
2013

Abstract

Background: Autosomal dominant polycystic kidney disease is a condition mainly character- ized by the progressive development and enlar- gement of cysts in each kidney. In this process a high rate of proliferation and apoptosis of tubu- lar cells has been documented and interpreted as a futile attempt of tissue repair. In considera- tion of the role of stem cells in reparative proc- esses we investigated the presence and local- ization of CD133 + CD24+ renal progenitors in renal ADPKD tissue and cells. Methods: Two normal kidneys and two ADPKD kidneys were examined. CD133 and CD24 expression was in- vestigated by confocal microscopy and immu- noblotting. Furthermore cystic isolated cells and cultured immortalized cells were characterized. Results: CD133 and CD24 have the same local- ization in ADPKD tissues and in normal kidneys: expression is restricted to a subset of epithelial cells (PEC) of Bowman’s capsule and to tubular cells in a focal and segmental pattern. Further- more, in ADPKD tissue, cysts diffusely express CD133 and CD24. According to a quantitative analysis in ADPKD tissue CD133 + CD24 + cells are statistically more expressed in tubules (p < 0.001) and less expressed in the Bowman’s capsule (p = 0.0016) compared to the same lo- calizations in control tissue. Conclusions: CD133 and CD24 antigens, typically expressed by renal epithelial progenitors, are more expressed in ADPKD tubules and highly expressed in ADPKD cysts. Whether CD133 and CD24 expression would signify renal progenitor recruitment or alternatively an expression pattern of the dedif- ferentiation of ADPKD cells remains unclear.
3
211
217
CD133 and CD24 expression in renal tissue of patients affected by autosomal dominant polcystic kidney disease / Lodi, Daniele; Ligabue, Giulia; Cavazzini, Fabrizio; Lupo, Valentina; Cappelli, Gianni; Magistroni, Riccardo. - In: STEM CELL DISCOVERY. - ISSN 2161-6760. - ELETTRONICO. - 3:(2013), pp. 211-217. [10.4236/scd.2013.34026]
Lodi, Daniele; Ligabue, Giulia; Cavazzini, Fabrizio; Lupo, Valentina; Cappelli, Gianni; Magistroni, Riccardo
File in questo prodotto:
File Dimensione Formato  
Lodi SCD 2013.pdf

accesso aperto

Descrizione: Articolo
Tipologia: Post-print dell'autore (bozza post referaggio)
Dimensione 2.93 MB
Formato Adobe PDF
2.93 MB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/1023332
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact