LONG-DISTANCE PATHWAYS OF DIFFUSION FOR DEXTRAN ALONG FIBER-BUNDLES IN BRAIN - RELEVANCE FOR VOLUME TRANSMISSION

TEXAS Red-labelled dextran with a mol. wt of 3000 g mol(-1), a marker for the extracellular space, was injected unilaterally into the neostriatum of adult rats (0.3-30 mu g mu(-1)) and its distribution evaluated 1 min to 5 h later. Diffusion in the neuropil was observed with clearance starting after 30 min. After 10-15 min strong labelling along the myelinated fibre bundles was observed in the entire neostriatum. After about 20 min the labelling along the fibres reached into the corpus callosum and the overlaying deep layers of the cerebral cortex. A marked cellular uptake and accumulation of labelled dextran was found in putative perivascular pericytes. Thus, in the living brain preferential extracellular fluid pathways for diffusion exist, especially along fibre bundles, which allow the exchange of chemical signals between two distant regions. These may represent extracellular fluid pathways for volume transmission.