In spite of the numerous reports implicating MafB transcription factor in the molecular control of monocyte-macrophage differentiation, the precise genetic program underlying this activity has been, to date, poorly understood. To clarify this issue, we planned a number of experiments that were mainly conducted on human primary macrophages. In this regard, a preliminary gene function study, based on MafB inactivation and over-expression, indicated MMP9 and IL-7R genes as possible targets of the investigated transcription factor. Bioinformatics analysis of their promoter regions disclosed the presence of several putative MARE elements and a combined approach of EMSA and luciferase assay subsequently demonstrated that expression of both genes is indeed activated by MafB through a direct transcription mechanism. Additional investigation, performed with similar procedures to elucidate the biological relevance of our observation, revealed that MafB is a downstream target of the IL-10/STAT3 signaling pathway, normally inducing the macrophage de-activation process. Taken together our data support the existence of a signaling cascade by which stimulation of macrophages with the IL-10 cytokine determines a sequential activation of STAT3 and MafB transcription factors, in turn leading to an up-regulated expression of MMP9 and IL-7R genes.
|Data di pubblicazione:||2014|
|Titolo:||MafB is a downstream target of the IL-10/STAT3 signaling pathway, involved in the regulation of macrophage de-activation.|
|Autore/i:||Gemelli C;Zanocco Marani T;Bicciato S;Mazza EM;Boraschi D;Salsi V;Zappavigna V;Parenti S;Selmi T;Tagliafico E;Ferrari S;Grande A|
|Digital Object Identifier (DOI):||10.1016/j.bbamcr.2014.01.021|
|Codice identificativo ISI:||WOS:000334476700014|
|Codice identificativo Scopus:||2-s2.0-84894497372|
|Codice identificativo Pubmed:||24472656|
|Citazione:||MafB is a downstream target of the IL-10/STAT3 signaling pathway, involved in the regulation of macrophage de-activation. / Gemelli C;Zanocco Marani T;Bicciato S;Mazza EM;Boraschi D;Salsi V;Zappavigna V;Parenti S;Selmi T;Tagliafico E;Ferrari S;Grande A. - In: BIOCHIMICA ET BIOPHYSICA ACTA. - ISSN 0006-3002. - ELETTRONICO. - 1843(2014), pp. 955-964.|
|Tipologia||Articolo su rivista|
I documenti presenti in Iris Unimore sono rilasciati con licenza Creative Commons Attribuzione - Non commerciale - Non opere derivate 3.0 Italia, salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris