Tomato (Solanum lycopersicum Mill.) is sensitive to chilling stress during all stages of plant development. Genetic variation for chilling tolerance exists between cultivated tomato and its related wild species, but intra-specific variation has not been thoroughly investigated so far. Seedlings of 63 tomato accessions were evaluated under low temperature and two contrasting cultivars were identified for the trait: Albenga and San Marzano, the former being more chillingtolerant. To clarify the molecular mechanisms of chilling tolerance in tomato, changes in candidate gene expressions in the two tomato genotypes were analysed, using quantitative RT-PCR. Candidate genes were chosen among those known to be induced by chilling and/or with putative roles in CBF/DREB and ROS-mediated pathways. Results show that besides a CBF regulon, whose function is conserved, ROS and C2H2-type zinc finger protein-mediated cold signalling pathways were also involved in chilling tolerance. Under the chilling stress, the up-regulation of respective transcripts was consistently higher in the chilling-tolerant genotype than in the chilling-sensitive ones.

Candidate gene expression profiling in two contrasting tomato cultivars under chilling stress / Caffagni, Alessandra; Pecchioni, Nicola; Francia, Enrico; D., Pagani; Milc, Justyna Anna. - In: BIOLOGIA PLANTARUM. - ISSN 0006-3134. - STAMPA. - 58:(2014), pp. 283-295. [10.1007/s10535-014-0403-z]

Candidate gene expression profiling in two contrasting tomato cultivars under chilling stress

CAFFAGNI, Alessandra;PECCHIONI, Nicola;FRANCIA, Enrico;MILC, Justyna Anna
2014

Abstract

Tomato (Solanum lycopersicum Mill.) is sensitive to chilling stress during all stages of plant development. Genetic variation for chilling tolerance exists between cultivated tomato and its related wild species, but intra-specific variation has not been thoroughly investigated so far. Seedlings of 63 tomato accessions were evaluated under low temperature and two contrasting cultivars were identified for the trait: Albenga and San Marzano, the former being more chillingtolerant. To clarify the molecular mechanisms of chilling tolerance in tomato, changes in candidate gene expressions in the two tomato genotypes were analysed, using quantitative RT-PCR. Candidate genes were chosen among those known to be induced by chilling and/or with putative roles in CBF/DREB and ROS-mediated pathways. Results show that besides a CBF regulon, whose function is conserved, ROS and C2H2-type zinc finger protein-mediated cold signalling pathways were also involved in chilling tolerance. Under the chilling stress, the up-regulation of respective transcripts was consistently higher in the chilling-tolerant genotype than in the chilling-sensitive ones.
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295
Candidate gene expression profiling in two contrasting tomato cultivars under chilling stress / Caffagni, Alessandra; Pecchioni, Nicola; Francia, Enrico; D., Pagani; Milc, Justyna Anna. - In: BIOLOGIA PLANTARUM. - ISSN 0006-3134. - STAMPA. - 58:(2014), pp. 283-295. [10.1007/s10535-014-0403-z]
Caffagni, Alessandra; Pecchioni, Nicola; Francia, Enrico; D., Pagani; Milc, Justyna Anna
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/1010721
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