Nematicidal effect of chestnut tannin solutions on potato cyst and root-knot nematodes

Two glasshouse pot experiments were carried out to evaluate the effect of chestnut tannins in the control of the potato cyst nematode Globodera rostochiensis patotype Ro1 and the root-knot nematode Meloidogyne javanica. Doses of 100, 250 and 450 g/m2 of tannins in aqueous solutions were applied to the soil at transplant or at transplant and two weeks later to verify their effect on nematode control. In both experiments tannin treatments were compared to untreated controls. Cysts were extracted from dried soil using the Fenwich can. Root-knot nematodes were extracted from roots by the Hussey and Barker’s method using 1% NaOCl aqueous solution and from soil processing 500 ml soil by the Coolen’s method. Data were subjected to analysis of variance and means compared by Least Significant Difference’s test. Significant reductions of G. rostochiensis number of cyst/100 g soil and eggs and juveniles/g soil and eggs and juveniles/g root and total M.javanica population density were observed in association with decreased reproduction rates.