BACKGROUND: Epstein Barr Virus (EBV) is responsible of many oncologic and non-oncologic diseases, including hemophagocytic syndrome in which red blood cells are phagocytosed by macrophages and B cells. EBV is reported to infect mainly B lymphocytes and epithelial cells; virus can be detected also in rare monocytes/macrophages, but its efficient replication in these cells has not been demonstrated.MATERIALS AND METHODS: EBV was obtained from P3RH1 cells stimulated with phorbol and butirate. A human monocytic cell line (THP-1) and an EBV negative B cell line (BJA-B) were infected with P3HR1- EBV strain: virus growth was monitored by IFA with a monoclonal antibody and by Real-Time PCR TaqMan. The effects of virus replication on phagocytosis of Candida albicans were evaluated by means of a double stain immunofluorescence assay. Intracellular killing of phagocytosed candida cells was measured by the colony forming unit inhibition assay.RESULTS AND CONCLUSION EBV can productively infect THP-1 monocyte cell line. EBV infection results in a significant increase in macrophage phagocytic activity versus Candida albicans as well as alteration of intracellular killing. Also BJA-B cells were stimulated in their phagocytic activity by EBV infection

Gene expression profiling on monocytes displaying Herpes Simplex virus 1-induced dysregulation of antifungal defences / Cermelli, Claudio; Cuoghi, A.; Orsi, Carlotta Francesca; Fantoni, L.; Blasi, Elisabetta. - STAMPA. - 1:(2008), pp. 39-39. ((Intervento presentato al convegno 8th National congress of the Italian Society of Virology tenutosi a Orvieto nel 21-23 settembre 2008.

Gene expression profiling on monocytes displaying Herpes Simplex virus 1-induced dysregulation of antifungal defences.

CERMELLI, Claudio;ORSI, Carlotta Francesca;BLASI, Elisabetta
2008-01-01

Abstract

BACKGROUND: Epstein Barr Virus (EBV) is responsible of many oncologic and non-oncologic diseases, including hemophagocytic syndrome in which red blood cells are phagocytosed by macrophages and B cells. EBV is reported to infect mainly B lymphocytes and epithelial cells; virus can be detected also in rare monocytes/macrophages, but its efficient replication in these cells has not been demonstrated.MATERIALS AND METHODS: EBV was obtained from P3RH1 cells stimulated with phorbol and butirate. A human monocytic cell line (THP-1) and an EBV negative B cell line (BJA-B) were infected with P3HR1- EBV strain: virus growth was monitored by IFA with a monoclonal antibody and by Real-Time PCR TaqMan. The effects of virus replication on phagocytosis of Candida albicans were evaluated by means of a double stain immunofluorescence assay. Intracellular killing of phagocytosed candida cells was measured by the colony forming unit inhibition assay.RESULTS AND CONCLUSION EBV can productively infect THP-1 monocyte cell line. EBV infection results in a significant increase in macrophage phagocytic activity versus Candida albicans as well as alteration of intracellular killing. Also BJA-B cells were stimulated in their phagocytic activity by EBV infection
8th National congress of the Italian Society of Virology
Orvieto
21-23 settembre 2008
Cermelli, Claudio; Cuoghi, A.; Orsi, Carlotta Francesca; Fantoni, L.; Blasi, Elisabetta
Gene expression profiling on monocytes displaying Herpes Simplex virus 1-induced dysregulation of antifungal defences / Cermelli, Claudio; Cuoghi, A.; Orsi, Carlotta Francesca; Fantoni, L.; Blasi, Elisabetta. - STAMPA. - 1:(2008), pp. 39-39. ((Intervento presentato al convegno 8th National congress of the Italian Society of Virology tenutosi a Orvieto nel 21-23 settembre 2008.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/646250
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