In this study we produced cationic SLN using stearicacid, stearylamine and protamine sulfate astransfection promoter, in order to improve SLN transfection capacity without the use of adjuvantsubstances. In fact, protamine is a cationic smallprotein with high arginine content that is FDAapproved for the parenteral administration. Since protamine is a nuclear proteinthat helps DNA packaging in sperm cells, it is also used as transfection acceleratorin the gene delivery.Therefore, in this work we optimized the protamineamount in SLN formulation in order to evaluate therole of this protein in the transfection activity ofcationic SLN.
The role of protamine in the gene delivery by cationic SLN on different cell lines / Vighi, Eleonora; Montanari, Monica; Ruozi, Barbara; Battini, Renata; Leo, Eliana Grazia. - STAMPA. - -:(2010), pp. ---. ((Intervento presentato al convegno 7th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology tenutosi a Malta nel Marzo 2010.
The role of protamine in the gene delivery by cationic SLN on different cell lines
VIGHI, Eleonora;MONTANARI, Monica;RUOZI, Barbara;BATTINI, Renata;LEO, Eliana Grazia
2010
Abstract
In this study we produced cationic SLN using stearicacid, stearylamine and protamine sulfate astransfection promoter, in order to improve SLN transfection capacity without the use of adjuvantsubstances. In fact, protamine is a cationic smallprotein with high arginine content that is FDAapproved for the parenteral administration. Since protamine is a nuclear proteinthat helps DNA packaging in sperm cells, it is also used as transfection acceleratorin the gene delivery.Therefore, in this work we optimized the protamineamount in SLN formulation in order to evaluate therole of this protein in the transfection activity ofcationic SLN.
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