Essential fatty acids (EFAs) belonging to -3 and -6 families play an important role in vivo, supporting the cardiovascular, reproductive, immune and nervous system functions; an inappropriate balance of these EFAs contributes to the development of diseases, while a proper balance helps maintain and even improve health.The purpose of this research was to study the levels of the principal -3 (ALA: n-lino-lenic acid; EPA: eicosapentaenoic acid; DPA: docosapentaenoic acid; DHA: docosa-hexaenoic acid) and -6 (LA: linoleic acid; AA: arachidonic acid) fatty acids in rat serum and tissues (liver, brain, adipose tissue) after a chronic treatment with flaxseed oil and with a dietetic oil, containing flaxseed oil, fish oil and sunflowers oil. The treatment was planned in order to give to rats the same ALA amount.The biological samples were subjected to liquid-liquid extraction, followed by derivatisation with p-bromophenacyl bromide and then analysed by HPLC/UV on a C18 reversed-phase column under gradient mobile phase conditions.Rats treated with flaxseed oil and dietetic oil showed enhanced levels of n-3 fatty acids compared to the correspondent control group. In serum, ALA levels were slightly higher in the dietetic oil group than in the flaxseed oil group; this fact is probably due to an enhanced absorption and also a decreased metabolism of ALA. The EPA, DPA and DHA serum levels, higher in the dietetic than in the flaxseed group, could derive both from ALA through its metabolism and also from EPA and DHA contained in the fish oil. Brain and adipose tissue showed EPA and DHA levels higher in the dietetic than in the flaxseed group, as occurred for serum samples. Concerning liver samples, the treatment with flaxseed or dietetic oil caused an enhancement for ALA, EPA and DPA levels, while no variations were found for DHA compared to control groups.The data obtained underline that the tested oils can enhance the levels of -3 fatty acids and therefore they could be successfully used in the dietetic treatment of lipid related diseases.
HPLC determination of fatty acid levels in rats after chronic supplementation of flaxseed oil and dietetic oil / Rustichelli, Cecilia; Avallone, Rossella; Campioli, Enrico; Braghiroli, Daniela; Baraldi, Mario. - STAMPA. - 1:(2009), pp. 133-133. (Intervento presentato al convegno RDPA 2009 tenutosi a Milano nel 9-12 settembre 2009).
HPLC determination of fatty acid levels in rats after chronic supplementation of flaxseed oil and dietetic oil.
RUSTICHELLI, Cecilia;AVALLONE, Rossella;CAMPIOLI, Enrico;BRAGHIROLI, Daniela;BARALDI, Mario
2009
Abstract
Essential fatty acids (EFAs) belonging to -3 and -6 families play an important role in vivo, supporting the cardiovascular, reproductive, immune and nervous system functions; an inappropriate balance of these EFAs contributes to the development of diseases, while a proper balance helps maintain and even improve health.The purpose of this research was to study the levels of the principal -3 (ALA: n-lino-lenic acid; EPA: eicosapentaenoic acid; DPA: docosapentaenoic acid; DHA: docosa-hexaenoic acid) and -6 (LA: linoleic acid; AA: arachidonic acid) fatty acids in rat serum and tissues (liver, brain, adipose tissue) after a chronic treatment with flaxseed oil and with a dietetic oil, containing flaxseed oil, fish oil and sunflowers oil. The treatment was planned in order to give to rats the same ALA amount.The biological samples were subjected to liquid-liquid extraction, followed by derivatisation with p-bromophenacyl bromide and then analysed by HPLC/UV on a C18 reversed-phase column under gradient mobile phase conditions.Rats treated with flaxseed oil and dietetic oil showed enhanced levels of n-3 fatty acids compared to the correspondent control group. In serum, ALA levels were slightly higher in the dietetic oil group than in the flaxseed oil group; this fact is probably due to an enhanced absorption and also a decreased metabolism of ALA. The EPA, DPA and DHA serum levels, higher in the dietetic than in the flaxseed group, could derive both from ALA through its metabolism and also from EPA and DHA contained in the fish oil. Brain and adipose tissue showed EPA and DHA levels higher in the dietetic than in the flaxseed group, as occurred for serum samples. Concerning liver samples, the treatment with flaxseed or dietetic oil caused an enhancement for ALA, EPA and DPA levels, while no variations were found for DHA compared to control groups.The data obtained underline that the tested oils can enhance the levels of -3 fatty acids and therefore they could be successfully used in the dietetic treatment of lipid related diseases.
Pubblicazioni consigliate
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris
