One of the primary events or response of human keratinocyte to irritant is the release of a variety of mediators. Among these, interleukin 1 alpha (IL-1alfa) has been shown to be increased following different stimuli induced by inflammatory agents or microbial endotoxins. Furthermore, it has been shown that IL-1 alfa secretion is triggered by exposure to tensides.To study the direct effects of irritants on keratinocytes without the involvement of inflammatory cells and the secondary effects of barrier disruption, we used an in vitro culture model.In this preliminary study, we investigated the in vitro release of IL-1 alfa produced by cultured normal human keratinocytes (NHK) treated with Sodium Lauryl Sulfate (SLS), an anionic detergent, at concentrations ranging from 0,00001 to 0,005. diluited in a protein free medium (KBM).After 1 h exposure, cells were washed and the medium was changed. After 24 h the supernatant was collected for Enzyme Linked Immunoassorbent Assay (ELISA).Results presented here are the mean of three independent experiments.Our results show that extracellular secretion of IL-1 alfa has an increasing trend with increasing SLS doses and exposure times.
Dose response curves of interleukin 1 alpha release by cultured normal human keratinocyte after variable exposure to different concentrations of SLS / Benassi, Luisa; Bertazzoni, Giorgia; Magnoni, Cristina; A., Di Nardo; Seidenari, Stefania. - STAMPA. - .:(1999), pp. 120-120. (Intervento presentato al convegno International Conference on In vitro cytotoxicity mechanisms tenutosi a Roma nel 25-27 gennaio).
Dose response curves of interleukin 1 alpha release by cultured normal human keratinocyte after variable exposure to different concentrations of SLS
BENASSI, Luisa;BERTAZZONI, Giorgia;MAGNONI, Cristina;SEIDENARI, Stefania
1999
Abstract
One of the primary events or response of human keratinocyte to irritant is the release of a variety of mediators. Among these, interleukin 1 alpha (IL-1alfa) has been shown to be increased following different stimuli induced by inflammatory agents or microbial endotoxins. Furthermore, it has been shown that IL-1 alfa secretion is triggered by exposure to tensides.To study the direct effects of irritants on keratinocytes without the involvement of inflammatory cells and the secondary effects of barrier disruption, we used an in vitro culture model.In this preliminary study, we investigated the in vitro release of IL-1 alfa produced by cultured normal human keratinocytes (NHK) treated with Sodium Lauryl Sulfate (SLS), an anionic detergent, at concentrations ranging from 0,00001 to 0,005. diluited in a protein free medium (KBM).After 1 h exposure, cells were washed and the medium was changed. After 24 h the supernatant was collected for Enzyme Linked Immunoassorbent Assay (ELISA).Results presented here are the mean of three independent experiments.Our results show that extracellular secretion of IL-1 alfa has an increasing trend with increasing SLS doses and exposure times.Pubblicazioni consigliate
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