Interleukin 1 alfa is involved in the biological response to irritants as one of the most important inflammatory mediators. The aim of the present study was to evaluate IL-1 alfa production after exposure to Sodium Lauryl Sulfate (SLS) from cultured human keratinocytes, representing a model which makes it possible to assess the in vitro effects of SLS on epidermal cells independently from the skin immune system and barrier alterations.Normal human keratinocytes from plastic surgery were grown in serum-free medium. At subconfluency, cells were treated with SLS doses ranging from 0.00001 to 0.005%, and after a one-hour exposure the medium was changed.At different time points, the supernatant was collected for ELISA, and cells were harvested for Western blot analysis of pro-IL -1 alfa and IL-1 alfa .Extracellular secretion of IL-1 alfa from keratinocytes was increased in a dose-dependent manner following SLS treatment. The release of IL-1alfa starts at 30 minutes after exposure to SLS (DL 50), reaching a maximum at 3 hours.Western blot analysis showed a down-regulation of pro IL-1 alfa levels at 1 hour, while IL-1 alfa levels remained unchanged.
Sodium lauryl sulphate causes IL-1α release as a marker of irritant response in normal human keratinocytes: dose response and time course curves / Benassi, Luisa; Bertazzoni, Giorgia; Magnoni, Cristina; A., Di Nardo; Euclidi, Emanuela; Seidenari, Stefania. - STAMPA. - .:(1999), pp. 15-15. (Intervento presentato al convegno 12th International Contact Dermatitis Symposium & 1st International Symposium on the Prevention of Occupational Skin Desease in Hairdressers tenutosi a San Francisco nel 14-18 ottobre).
Sodium lauryl sulphate causes IL-1α release as a marker of irritant response in normal human keratinocytes: dose response and time course curves.
BENASSI, Luisa;BERTAZZONI, Giorgia;MAGNONI, Cristina;EUCLIDI, Emanuela;SEIDENARI, Stefania
1999
Abstract
Interleukin 1 alfa is involved in the biological response to irritants as one of the most important inflammatory mediators. The aim of the present study was to evaluate IL-1 alfa production after exposure to Sodium Lauryl Sulfate (SLS) from cultured human keratinocytes, representing a model which makes it possible to assess the in vitro effects of SLS on epidermal cells independently from the skin immune system and barrier alterations.Normal human keratinocytes from plastic surgery were grown in serum-free medium. At subconfluency, cells were treated with SLS doses ranging from 0.00001 to 0.005%, and after a one-hour exposure the medium was changed.At different time points, the supernatant was collected for ELISA, and cells were harvested for Western blot analysis of pro-IL -1 alfa and IL-1 alfa .Extracellular secretion of IL-1 alfa from keratinocytes was increased in a dose-dependent manner following SLS treatment. The release of IL-1alfa starts at 30 minutes after exposure to SLS (DL 50), reaching a maximum at 3 hours.Western blot analysis showed a down-regulation of pro IL-1 alfa levels at 1 hour, while IL-1 alfa levels remained unchanged.Pubblicazioni consigliate
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