Several microorganisms, part of the microbial communities' present in soil and plant (as endophytes or epiphytes), play a significant role in agro-environments as plant growth promoters, control of plant diseases, facilitation of nutrients uptake by plants. Understanding the mode of action of such microbes (bacteria, yeasts, fungi) and their relationship with crop plants will help to focus on selected microorganisms, with the scope to develop innovative products (Microbials) to be used in sustainable crop production, gaining a positive impact on agricultural environments in terms of microbial diversity and stability. The research focuses on searching microbial candidates in agricultural and natural environments suitable for crop management as biocontrol agents or plant growth promoters. The Streptomyces sp. strain SA51 and Pseudomonas sp. strain PT65 used in the present study were previously studied extensively to evaluate their in vitro plant growth promoting (PGP) and bio-control activity against various bacterial and fungal phytopathogens (AMI-2017, pp 222). Here, we characterized both the strains SA51 and PT65 for their colonization ability, plant growth promotion and protection against tomato spot disease caused by Xanthomonas vesicatoria on tomato (Solanum lycopersicum) as model plant. In this study, direct inhibitory action against X. vesicatoria by the bacterized tomato plants showed significantly good plant growth, particularly in aerial parts as compared to un-bacterized controls. In addition, benefit was seen in inoculated healthy plants in terms of increase in dry weight and length of roots and shoots as compared to the uninoculated controls. The mechanism of biocontrol also involved induction of plant defense response. Endophytic colonization of the streptomycetes was confirmed by both PCR amplification applied to different plant segments and its visual observation with a confocal microscopy, after its transformation with green fluorescent protein (gfp); gfp tagged Streptomyces strain SA51 showed profuse colonization in roots, collar and aerial parts of tomato plants.
Endophytic colonization of tomato plants by plant growth promoting rhizobacteria / Vurukonda, Sai Shiva Krishna Prasa; Stefani, Emilio. - 1:(2018), pp. 56-56. (Intervento presentato al convegno 59th Annual Conference of Association of Microbiologists of India & International Symposium on Host-Pathogen Interactions tenutosi a Hyderabad, India nel 9-12 December, 2018).
Endophytic colonization of tomato plants by plant growth promoting rhizobacteria
Vurukonda, Sai Shiva Krishna PrasaWriting – Original Draft Preparation
;Stefani, Emilio
Conceptualization
2018
Abstract
Several microorganisms, part of the microbial communities' present in soil and plant (as endophytes or epiphytes), play a significant role in agro-environments as plant growth promoters, control of plant diseases, facilitation of nutrients uptake by plants. Understanding the mode of action of such microbes (bacteria, yeasts, fungi) and their relationship with crop plants will help to focus on selected microorganisms, with the scope to develop innovative products (Microbials) to be used in sustainable crop production, gaining a positive impact on agricultural environments in terms of microbial diversity and stability. The research focuses on searching microbial candidates in agricultural and natural environments suitable for crop management as biocontrol agents or plant growth promoters. The Streptomyces sp. strain SA51 and Pseudomonas sp. strain PT65 used in the present study were previously studied extensively to evaluate their in vitro plant growth promoting (PGP) and bio-control activity against various bacterial and fungal phytopathogens (AMI-2017, pp 222). Here, we characterized both the strains SA51 and PT65 for their colonization ability, plant growth promotion and protection against tomato spot disease caused by Xanthomonas vesicatoria on tomato (Solanum lycopersicum) as model plant. In this study, direct inhibitory action against X. vesicatoria by the bacterized tomato plants showed significantly good plant growth, particularly in aerial parts as compared to un-bacterized controls. In addition, benefit was seen in inoculated healthy plants in terms of increase in dry weight and length of roots and shoots as compared to the uninoculated controls. The mechanism of biocontrol also involved induction of plant defense response. Endophytic colonization of the streptomycetes was confirmed by both PCR amplification applied to different plant segments and its visual observation with a confocal microscopy, after its transformation with green fluorescent protein (gfp); gfp tagged Streptomyces strain SA51 showed profuse colonization in roots, collar and aerial parts of tomato plants.
Pubblicazioni consigliate
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris
