A process for preserving cultured epidermal sheets by incubating the sheets at room temperature in media with a glycerol or dimethylsulfoxide cryopreservant for a predetermined period of time and then freezing the cultured sheets. The cooling gradient is characterized as having a lower initial rate of cooling followed by a higher rate of cooling.
Cryopreserved in vitro cultured epithelial tissue and method / Cancedda, R.; De Luca, Michele.
Cryopreserved in vitro cultured epithelial tissue and method
DE LUCA, Michele
1991
Abstract
A process for preserving cultured epidermal sheets by incubating the sheets at room temperature in media with a glycerol or dimethylsulfoxide cryopreservant for a predetermined period of time and then freezing the cultured sheets. The cooling gradient is characterized as having a lower initial rate of cooling followed by a higher rate of cooling.
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