A process for preserving cultured epithelial sheets by incubating the sheets at room temperature in media with a glycerol or dimethylsulfoxide as cryopreservant for a predetermined period of time and then freezing the cultured sheets. The cooling gradient is characterized as having a lower initial rate of cooling followed by a higher rate of cooling.
Method for preserving transplantable sheets of epithelium cultured in vitro / Cancedda, R.; De Luca, Michele. - (1987 Jun 23).
Method for preserving transplantable sheets of epithelium cultured in vitro
DE LUCA, Michele
1987
Abstract
A process for preserving cultured epithelial sheets by incubating the sheets at room temperature in media with a glycerol or dimethylsulfoxide as cryopreservant for a predetermined period of time and then freezing the cultured sheets. The cooling gradient is characterized as having a lower initial rate of cooling followed by a higher rate of cooling.
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