HR-MAS NMR spectroscopy was employed to monitor the metabolic profiles of Modena BioBank breast samples over one year of freezing at -80 °C. The study includes 22 adult female patients living in Modena and its hinterland, who underwent total mastectomy or quadrantectomy in 2011 - 2012. Variations occur, especially affecting phosphocholine and choline. This is not a trivial finding, since many studies base the distinction between neoplastic and healthy tissues or the assessment of tumor grade on the analysis of choline metabolites [1,2]. Despite the changes observed, we established that the diagnostic power of the HR-MAS NMR spectra of frozen samples is preserved, at least as far as the distinction between neoplastic and healthy samples is concerned. Lactate, phosphocholine, phosphoethanolamine, taurine, myo-inositol and glucose are biomarkers that can be used to distinguish healthy from neoplastic tissues, whereas some metabolite ratios, such as Lac+PE+Tau/Glc+Myo, seem to have even higher discrimination potential. Fig. 1. Scores plot of PCA on neoplastic (crosses) vs healthy (triangles) samples. Loadings profiles (right) of PC2 and PC3. References [1]

ASSESSMENT OF FREEZING EFFECTS AND DIAGNOSTIC POTENTIAL OF BIOBANK HEALTHY AND NEOPLASTIC BREAST TISSUES THROUGH HR-MAS NMR SPECTROSCOPY / Mucci, Adele; Schenetti, Luisa; Maiorana, Antonino; Bettelli, Stefania Raffaella; REGGIANI BONETTI, Luca; V., Righi. - ELETTRONICO. - 1:(2014), pp. OC31-OC31. ((Intervento presentato al convegno XVIII Convegno Nazionale delle Risonanze Magnetiche tenutosi a Bari nel 22-24 settembre 2014.

ASSESSMENT OF FREEZING EFFECTS AND DIAGNOSTIC POTENTIAL OF BIOBANK HEALTHY AND NEOPLASTIC BREAST TISSUES THROUGH HR-MAS NMR SPECTROSCOPY

MUCCI, Adele;SCHENETTI, Luisa;MAIORANA, Antonino;BETTELLI, Stefania Raffaella;REGGIANI BONETTI, Luca;
2014

Abstract

HR-MAS NMR spectroscopy was employed to monitor the metabolic profiles of Modena BioBank breast samples over one year of freezing at -80 °C. The study includes 22 adult female patients living in Modena and its hinterland, who underwent total mastectomy or quadrantectomy in 2011 - 2012. Variations occur, especially affecting phosphocholine and choline. This is not a trivial finding, since many studies base the distinction between neoplastic and healthy tissues or the assessment of tumor grade on the analysis of choline metabolites [1,2]. Despite the changes observed, we established that the diagnostic power of the HR-MAS NMR spectra of frozen samples is preserved, at least as far as the distinction between neoplastic and healthy samples is concerned. Lactate, phosphocholine, phosphoethanolamine, taurine, myo-inositol and glucose are biomarkers that can be used to distinguish healthy from neoplastic tissues, whereas some metabolite ratios, such as Lac+PE+Tau/Glc+Myo, seem to have even higher discrimination potential. Fig. 1. Scores plot of PCA on neoplastic (crosses) vs healthy (triangles) samples. Loadings profiles (right) of PC2 and PC3. References [1]
XVIII Convegno Nazionale delle Risonanze Magnetiche
Bari
22-24 settembre 2014
Mucci, Adele; Schenetti, Luisa; Maiorana, Antonino; Bettelli, Stefania Raffaella; REGGIANI BONETTI, Luca; V., Righi
ASSESSMENT OF FREEZING EFFECTS AND DIAGNOSTIC POTENTIAL OF BIOBANK HEALTHY AND NEOPLASTIC BREAST TISSUES THROUGH HR-MAS NMR SPECTROSCOPY / Mucci, Adele; Schenetti, Luisa; Maiorana, Antonino; Bettelli, Stefania Raffaella; REGGIANI BONETTI, Luca; V., Righi. - ELETTRONICO. - 1:(2014), pp. OC31-OC31. ((Intervento presentato al convegno XVIII Convegno Nazionale delle Risonanze Magnetiche tenutosi a Bari nel 22-24 settembre 2014.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/1038919
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