Considering the relevant antiproliferative activity of polyacetylenes/polyenes isolated from lipophilic root extracts of Echinacea pallida Nutt. (Nutt.) (Asteraceae family) and the chemopreventive action of caffeic acid derivatives identified in the hydrophilic fraction of this plant, this study provides an innovative and reliable method for the complete fingerprinting of these secondary metabolites by means of HPLC-UV/DAD, HPLC-ESI-MS and MS/MS. A relevant novel aspect of the proposed technique is that MS data, obtained by ion trap and triple quadrupole mass analyzers, made the peak identification very reliable. The fragmentation patterns of the bioactive compounds are discussed in detail in the present work. The oxidation mechanism of monocarbonyl acetylenes is shown for the first time using MS data. The practical applicability of the technique is demonstrated by the quantitative analysis of E. pallida root extracts and natural products commercially available to provide reliable chromatographic fingerprints of their bioactive secondary metabolites.
Advanced chromatographic techniques for metabolite fingerprinting of natural compounds with antioxidant and antiproliferative activity / Pellati, Federica. - STAMPA. - Fall 2013:(2013), pp. 22-23. (Intervento presentato al convegno Young Scientist Award Symposium, 246th ACS National Meeting & Exposition tenutosi a Indianapolis (USA) nel 8-12 Settembre 2013).
Advanced chromatographic techniques for metabolite fingerprinting of natural compounds with antioxidant and antiproliferative activity
PELLATI, Federica
2013
Abstract
Considering the relevant antiproliferative activity of polyacetylenes/polyenes isolated from lipophilic root extracts of Echinacea pallida Nutt. (Nutt.) (Asteraceae family) and the chemopreventive action of caffeic acid derivatives identified in the hydrophilic fraction of this plant, this study provides an innovative and reliable method for the complete fingerprinting of these secondary metabolites by means of HPLC-UV/DAD, HPLC-ESI-MS and MS/MS. A relevant novel aspect of the proposed technique is that MS data, obtained by ion trap and triple quadrupole mass analyzers, made the peak identification very reliable. The fragmentation patterns of the bioactive compounds are discussed in detail in the present work. The oxidation mechanism of monocarbonyl acetylenes is shown for the first time using MS data. The practical applicability of the technique is demonstrated by the quantitative analysis of E. pallida root extracts and natural products commercially available to provide reliable chromatographic fingerprints of their bioactive secondary metabolites.
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