Purpose: To explore the potential benefit provided by an organically modified phyllosilicate in the problematic management of the Helicobacter pylori gastric infection that is one of the most prevalent infectious disease worldwide. Methods: An organically modified layered-silicate or nanoclay was produced by the intercalation of tetracycline (TC) into the interlayer of montmorillonite (MM) under two different pH reaction conditions (pH 3.0 and 8.7). MM-TC organoclays were characterized by EDX, XRD, FT-IR, DSC, drug content and in vitro release. Moreover, the organoclays were evaluated in vitro for mucoadhesiveness and buoyancy ability upon suspension in a sodium alginate hydrogel. Results: The reaction between clay and TC led to a complete MM cation (Na+ and Ca2+) exchange process, to an increase of MM characteristic interlayer spacing as well as to an involvement of NHR3+ group of TC, regardless of the medium pH value, as shown by EDX, XRD and FT-IR analyses. However, MM-TC obtained under alkaline condition determined a lower sorption of TC into clay in comparison with MM-TC obtained in acidic condition as well as a drug fraction weakly linked to MM and quickly released. Both the organoclays exhibited good mucoadhesion properties to mucin as well as to porcine gastric mucosa and good floatability in a sodium alginate water solution. Conclusion: TC intercalated into MM nanoplatelets and dispersed in an alginate hydrogel could represent an advantageous formulation allowing the antibiotic to distribute homogeneously on the gastric mucosa and to diffuse gradually into the gastric mucus layer where the microorganism is localized.

Tetracycline-Montmorillonite nanoclay for the treatment of H. pylori infection / Iannuccelli, Valentina; Montorsi, Monia; Sacchetti, Francesca; Maretti, Eleonora; Coppi, Gilberto; Leo, Eliana Grazia. - STAMPA. - ND:(2013), pp. 114-114. (Intervento presentato al convegno 3rd Conference on Innovation in drug delivery: Advances in local drug delivery tenutosi a Pisa nel 22-25 settembre 2013).

Tetracycline-Montmorillonite nanoclay for the treatment of H. pylori infection

IANNUCCELLI, Valentina;MONTORSI, Monia;SACCHETTI, FRANCESCA;MARETTI, ELEONORA;COPPI, Gilberto;LEO, Eliana Grazia
2013

Abstract

Purpose: To explore the potential benefit provided by an organically modified phyllosilicate in the problematic management of the Helicobacter pylori gastric infection that is one of the most prevalent infectious disease worldwide. Methods: An organically modified layered-silicate or nanoclay was produced by the intercalation of tetracycline (TC) into the interlayer of montmorillonite (MM) under two different pH reaction conditions (pH 3.0 and 8.7). MM-TC organoclays were characterized by EDX, XRD, FT-IR, DSC, drug content and in vitro release. Moreover, the organoclays were evaluated in vitro for mucoadhesiveness and buoyancy ability upon suspension in a sodium alginate hydrogel. Results: The reaction between clay and TC led to a complete MM cation (Na+ and Ca2+) exchange process, to an increase of MM characteristic interlayer spacing as well as to an involvement of NHR3+ group of TC, regardless of the medium pH value, as shown by EDX, XRD and FT-IR analyses. However, MM-TC obtained under alkaline condition determined a lower sorption of TC into clay in comparison with MM-TC obtained in acidic condition as well as a drug fraction weakly linked to MM and quickly released. Both the organoclays exhibited good mucoadhesion properties to mucin as well as to porcine gastric mucosa and good floatability in a sodium alginate water solution. Conclusion: TC intercalated into MM nanoplatelets and dispersed in an alginate hydrogel could represent an advantageous formulation allowing the antibiotic to distribute homogeneously on the gastric mucosa and to diffuse gradually into the gastric mucus layer where the microorganism is localized.
2013
3rd Conference on Innovation in drug delivery: Advances in local drug delivery
Pisa
22-25 settembre 2013
Iannuccelli, Valentina; Montorsi, Monia; Sacchetti, Francesca; Maretti, Eleonora; Coppi, Gilberto; Leo, Eliana Grazia
Tetracycline-Montmorillonite nanoclay for the treatment of H. pylori infection / Iannuccelli, Valentina; Montorsi, Monia; Sacchetti, Francesca; Maretti, Eleonora; Coppi, Gilberto; Leo, Eliana Grazia. - STAMPA. - ND:(2013), pp. 114-114. (Intervento presentato al convegno 3rd Conference on Innovation in drug delivery: Advances in local drug delivery tenutosi a Pisa nel 22-25 settembre 2013).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/977700
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