A method for the preservation of in vitro expanded epithelial sheets which comprises the incubation of said sheets in a cryopreserving solution characterized by lack of volume increase at the liquid-solid phase transition. The cryopreserving solutions comprise: a) polyethylene glycols having molecular weight not higher than 20 KD; b) at least one cross-linking agent selected from polyols, polyamines, mono- or oligosaccharides, polyethyleneglycols having molecular weight lower than 1 KD .
CRYOPROTECTIVE AQUEOUS SOLUTIONS USEFUL FOR THE PRESERVATION OF IN VlTRO CULTURED EPITHELIAL SHEETS / G., Geraci; M., DE ROSA; M., Rossi; R., Cancedda; DE LUCA, Michele; Pellegrini, Graziella. - (1993 Dec 01).
CRYOPROTECTIVE AQUEOUS SOLUTIONS USEFUL FOR THE PRESERVATION OF IN VlTRO CULTURED EPITHELIAL SHEETS
DE LUCA, Michele;PELLEGRINI, Graziella
1993
Abstract
A method for the preservation of in vitro expanded epithelial sheets which comprises the incubation of said sheets in a cryopreserving solution characterized by lack of volume increase at the liquid-solid phase transition. The cryopreserving solutions comprise: a) polyethylene glycols having molecular weight not higher than 20 KD; b) at least one cross-linking agent selected from polyols, polyamines, mono- or oligosaccharides, polyethyleneglycols having molecular weight lower than 1 KD .
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