A rapid and sensitive liquid chromatography/tandem mass spectrometry (LC–MS/MS) method has been developed for the simultaneous measurement of adenosine (ADE), dopamine (DA), acetylcholine (ACh) and 5-hydroxytryptamine (5-HT) in mouse brain microdialysates. High method sensitivity (LLOQ of 0.05 nM) was achieved by optimization of chromatographic and mass spectrometric parameters. The method was fully validated for its sensitivity, selectivity, matrix effect and stability. The LC–MS/MS method was successfully applied to evaluate the effect of the systemic administration of cocaine or amphetamine on the extracellular levels of ADE, DA, ACh and 5-HT in the mouse nucleus accumbens by microdialysis.

Simultaneous measurement of adenosine, dopamine, acetylcholine and 5-hydroxytryptamine in cerebral mice microdialysis samples by LC–ESI-MS/MS / Cannazza, Giuseppe; Carrozzo, Mm; Cazzato, As; Bretis, Im; Troisi, L; Parenti, Carlo; Braghiroli, Daniela; Guiducci, Stefania; Zoli, Michele. - In: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. - ISSN 0731-7085. - STAMPA. - 71:(2012), pp. 183-186. [10.1016/j.jpba.2012.08.004]

Simultaneous measurement of adenosine, dopamine, acetylcholine and 5-hydroxytryptamine in cerebral mice microdialysis samples by LC–ESI-MS/MS

CANNAZZA, Giuseppe;PARENTI, Carlo;BRAGHIROLI, Daniela;GUIDUCCI, Stefania;ZOLI, Michele
2012

Abstract

A rapid and sensitive liquid chromatography/tandem mass spectrometry (LC–MS/MS) method has been developed for the simultaneous measurement of adenosine (ADE), dopamine (DA), acetylcholine (ACh) and 5-hydroxytryptamine (5-HT) in mouse brain microdialysates. High method sensitivity (LLOQ of 0.05 nM) was achieved by optimization of chromatographic and mass spectrometric parameters. The method was fully validated for its sensitivity, selectivity, matrix effect and stability. The LC–MS/MS method was successfully applied to evaluate the effect of the systemic administration of cocaine or amphetamine on the extracellular levels of ADE, DA, ACh and 5-HT in the mouse nucleus accumbens by microdialysis.
2012
71
183
186
Simultaneous measurement of adenosine, dopamine, acetylcholine and 5-hydroxytryptamine in cerebral mice microdialysis samples by LC–ESI-MS/MS / Cannazza, Giuseppe; Carrozzo, Mm; Cazzato, As; Bretis, Im; Troisi, L; Parenti, Carlo; Braghiroli, Daniela; Guiducci, Stefania; Zoli, Michele. - In: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. - ISSN 0731-7085. - STAMPA. - 71:(2012), pp. 183-186. [10.1016/j.jpba.2012.08.004]
Cannazza, Giuseppe; Carrozzo, Mm; Cazzato, As; Bretis, Im; Troisi, L; Parenti, Carlo; Braghiroli, Daniela; Guiducci, Stefania; Zoli, Michele
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/812725
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