Heterogeneous nuclear RNA was extracted from normal PHA-stimulated human lymphocytes and acute myeloid leukemia blast cells. Experiments were performed to determine the hybridization kinetics of these RNA's to human DNA. The best least squares solutions indicate in the hybridization reaction of both normal and leukemic RNA two main components. For leukemic cell RNA the rate constants of both components were significantly different from that of normal cell RNA. In particular, the difference between the rate constants of the second lower component suggests that the slowly hybridizing sequences in leukemic cell RNA have a degree of repetition higher than of the corresponding sequences of normal cell RNA.

Kinetics of hybridization to human DNA of heterogeneous nuclear RNA isolated from normal human lymphoblasts and acute leukemia blast cells / Torelli, Giuseppe; Narni, Franco; Franchini, G; Donelli, A; Ferrari, Sergio; Calabretta, Bruno; Torelli, Umberto; Bosi, Paolo. - In: MOLECULAR BIOLOGY REPORTS. - ISSN 0301-4851. - STAMPA. - 5:(1979), pp. 181-184.

Kinetics of hybridization to human DNA of heterogeneous nuclear RNA isolated from normal human lymphoblasts and acute leukemia blast cells.

TORELLI, Giuseppe;NARNI, Franco;FERRARI, Sergio;CALABRETTA, Bruno;TORELLI, Umberto;BOSI, Paolo
1979

Abstract

Heterogeneous nuclear RNA was extracted from normal PHA-stimulated human lymphocytes and acute myeloid leukemia blast cells. Experiments were performed to determine the hybridization kinetics of these RNA's to human DNA. The best least squares solutions indicate in the hybridization reaction of both normal and leukemic RNA two main components. For leukemic cell RNA the rate constants of both components were significantly different from that of normal cell RNA. In particular, the difference between the rate constants of the second lower component suggests that the slowly hybridizing sequences in leukemic cell RNA have a degree of repetition higher than of the corresponding sequences of normal cell RNA.
1979
5
181
184
Kinetics of hybridization to human DNA of heterogeneous nuclear RNA isolated from normal human lymphoblasts and acute leukemia blast cells / Torelli, Giuseppe; Narni, Franco; Franchini, G; Donelli, A; Ferrari, Sergio; Calabretta, Bruno; Torelli, Umberto; Bosi, Paolo. - In: MOLECULAR BIOLOGY REPORTS. - ISSN 0301-4851. - STAMPA. - 5:(1979), pp. 181-184.
Torelli, Giuseppe; Narni, Franco; Franchini, G; Donelli, A; Ferrari, Sergio; Calabretta, Bruno; Torelli, Umberto; Bosi, Paolo
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/746511
Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 0
  • ???jsp.display-item.citation.isi??? 0
social impact