Aim of study: Plasma Homocysteine (HCY) is free or bound to plasma proteins (PbHCY). A moderate increase of HCY is a risk factor for vascular disease, even though by mechanism not yet known: it may depend on oxidative HCY-induced modification of lipoproteins (LP). The present study aims to evaluate the LP-HCY binding in vivo. Materials and methods: 53 subjects (mean age 7120 yr) were studied. HCY was measured by HPLC method. Plasma lipoprotein fractions were separated by sequential ultracentrifugation. Single fraction purity was tested on agarose gel electrophoresis. HCY bound to single plasma protein fraction was calculated as difference between total and free HCY. Results: Pb-HCY (mean value: 19.4±2 nmol/ml) results on average about 80% of total plasma HCY (mean values 24.2±2.5 nmol/ml). Distribution (absolute and percentage mean values) of Pb-HCY (nmol/ml) among the different fractions and single fraction HCY/protein ratio (nmol of HCY/mg of protein) are shown in the table. FractionsPb-HCY % of Pb-HCYHCY/protein ratio VLDL1 ± 0.65.10.76 ± 0.4LDL1.5 ± 0.57.70.52 ± 0.3HDL1.9 ± 0.59.70.11 ± 0.1LFF*15.1 ± 277.5< 0.1*LFF= Lipoprotein free fraction (density > 1.21 g/ml) Conclusions: A fraction of plasma HCY circulates bound to LP (about 23% of Pb-HCY); if single Pb-HCY fraction values are considered in terms of ratio between Pb-HCY (nmol) and mg of protein, VLDL and LDL fractions show the highest “affinity” for HCY. These results might explain the damaging effect of HCY on the vessel wall.

Distribution of homocysteine in plasma lipoproteins / Ventura, Paolo; Panini, Rossana; P., Paolello; G., Scarpetta; Salvioli, Gianfranco. - In: ATHEROSCLEROSIS. - ISSN 1879-1484. - STAMPA. - 135 (suppl 1):(1997), pp. S20-S20. (Intervento presentato al convegno XI National congress of the Italian Society for the study of atherosclerosis tenutosi a Vibo Valentia nel 1-2 dicembre 1997).

Distribution of homocysteine in plasma lipoproteins

VENTURA, Paolo;PANINI, Rossana;SALVIOLI, Gianfranco
1997

Abstract

Aim of study: Plasma Homocysteine (HCY) is free or bound to plasma proteins (PbHCY). A moderate increase of HCY is a risk factor for vascular disease, even though by mechanism not yet known: it may depend on oxidative HCY-induced modification of lipoproteins (LP). The present study aims to evaluate the LP-HCY binding in vivo. Materials and methods: 53 subjects (mean age 7120 yr) were studied. HCY was measured by HPLC method. Plasma lipoprotein fractions were separated by sequential ultracentrifugation. Single fraction purity was tested on agarose gel electrophoresis. HCY bound to single plasma protein fraction was calculated as difference between total and free HCY. Results: Pb-HCY (mean value: 19.4±2 nmol/ml) results on average about 80% of total plasma HCY (mean values 24.2±2.5 nmol/ml). Distribution (absolute and percentage mean values) of Pb-HCY (nmol/ml) among the different fractions and single fraction HCY/protein ratio (nmol of HCY/mg of protein) are shown in the table. FractionsPb-HCY % of Pb-HCYHCY/protein ratio VLDL1 ± 0.65.10.76 ± 0.4LDL1.5 ± 0.57.70.52 ± 0.3HDL1.9 ± 0.59.70.11 ± 0.1LFF*15.1 ± 277.5< 0.1*LFF= Lipoprotein free fraction (density > 1.21 g/ml) Conclusions: A fraction of plasma HCY circulates bound to LP (about 23% of Pb-HCY); if single Pb-HCY fraction values are considered in terms of ratio between Pb-HCY (nmol) and mg of protein, VLDL and LDL fractions show the highest “affinity” for HCY. These results might explain the damaging effect of HCY on the vessel wall.
1997
135 (suppl 1)
S20
S20
Ventura, Paolo; Panini, Rossana; P., Paolello; G., Scarpetta; Salvioli, Gianfranco
Distribution of homocysteine in plasma lipoproteins / Ventura, Paolo; Panini, Rossana; P., Paolello; G., Scarpetta; Salvioli, Gianfranco. - In: ATHEROSCLEROSIS. - ISSN 1879-1484. - STAMPA. - 135 (suppl 1):(1997), pp. S20-S20. (Intervento presentato al convegno XI National congress of the Italian Society for the study of atherosclerosis tenutosi a Vibo Valentia nel 1-2 dicembre 1997).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/617256
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