The p5 promoter region of the adeno-associated virus type 2 (AAV-2) rep gene has been described as essential for Rep-mediated site-specific integration (RMSSI) of plasmid sequences in human chromosome 19. We report here that insertion of a full-length or minimal p5 element between the viral inverted terminal repeats does not significantly increase RMSSI of a recombinant AAV (rAAV) vector after infection of growth-arrested or proliferating human cells. This result suggests that the p5 element may not improve RMSSI of rAAV vectors in vivo.
Relative influence of the adeno-associated virus (AAV) type 2 p5 element for recombinant AAV vector site-specific integration / Guilbaud, M; Chadeuf, G; Avolio, F; François, A; Moullier, P; Recchia, Alessandra; Salvetti, A.. - In: JOURNAL OF VIROLOGY. - ISSN 0022-538X. - STAMPA. - 82:5(2008), pp. 2590-2593. [10.1128/JVI.01956-07]
Relative influence of the adeno-associated virus (AAV) type 2 p5 element for recombinant AAV vector site-specific integration
RECCHIA, Alessandra;
2008
Abstract
The p5 promoter region of the adeno-associated virus type 2 (AAV-2) rep gene has been described as essential for Rep-mediated site-specific integration (RMSSI) of plasmid sequences in human chromosome 19. We report here that insertion of a full-length or minimal p5 element between the viral inverted terminal repeats does not significantly increase RMSSI of a recombinant AAV (rAAV) vector after infection of growth-arrested or proliferating human cells. This result suggests that the p5 element may not improve RMSSI of rAAV vectors in vivo.
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