The objective of this clinical trial was the analysis of 2 methods for engineering of autologous bone grafts for maxillary sinus augmentation with secondary implant placement. Group 1 (8 patients, 12 sinuses): cells of mandibular periosteum were cultured in a good manufacturing practice laboratory (2 weeks) with autologous serum and then transferred onto a collagen matrix. After another week, these composites were transplanted into the sinuses. In group 2A (2 patients, 3 sinuses), cells of maxillary bone were cultivated with autologous serum for 2 weeks, seeded onto natural bone mineral (NBM, diameter [Ø] = 8 mm) blocks, and cultivated for another 1.5 months. These composites were transplanted into the sinuses. Group 2B (control, 3 patients, 5 sinuses) received NBM blocks alone. In the course of implant placement 6 (group 1) and 8 (group 2) months later, core biopsy were taken. Clinical follow-up period was 1 to 2.5 years in group 1 and approximately 7 years in groups 2A and 2B. New vital bone was found in all cases at median densities of 38\% (n = 12) in group 1, 32\% in group 2A (n = 3), and 25\% in group 2B (n = 5). Differences between group 1 and 2B as well as 2A and 2B were statistically significant ( p = 0.025). No adverse effects were seen. All methods described were capable of creating new bone tissue with sufficient stability for successful implant placement.

Two techniques for the preparation of cell-scaffold constructs suitable for sinus augmentation: steps into clinical application / Ingo N., Springer; Pier F., Nocini; Karl A., Schlegel; Daniele De, Santis; Jung, Park; Patrick H., Warnke; Hendrik, Terheyden; Robert, Zimmermann; Chiarini, Luigi; Klaus, Gardner; Francesca, Ferrari; Jörg, Wiltfang. - In: TISSUE ENGINEERING. - ISSN 1076-3279. - STAMPA. - 12:(2006), pp. 2649-2656. [10.1089/ten.2006.12.2649]

Two techniques for the preparation of cell-scaffold constructs suitable for sinus augmentation: steps into clinical application.

CHIARINI, Luigi;
2006

Abstract

The objective of this clinical trial was the analysis of 2 methods for engineering of autologous bone grafts for maxillary sinus augmentation with secondary implant placement. Group 1 (8 patients, 12 sinuses): cells of mandibular periosteum were cultured in a good manufacturing practice laboratory (2 weeks) with autologous serum and then transferred onto a collagen matrix. After another week, these composites were transplanted into the sinuses. In group 2A (2 patients, 3 sinuses), cells of maxillary bone were cultivated with autologous serum for 2 weeks, seeded onto natural bone mineral (NBM, diameter [Ø] = 8 mm) blocks, and cultivated for another 1.5 months. These composites were transplanted into the sinuses. Group 2B (control, 3 patients, 5 sinuses) received NBM blocks alone. In the course of implant placement 6 (group 1) and 8 (group 2) months later, core biopsy were taken. Clinical follow-up period was 1 to 2.5 years in group 1 and approximately 7 years in groups 2A and 2B. New vital bone was found in all cases at median densities of 38\% (n = 12) in group 1, 32\% in group 2A (n = 3), and 25\% in group 2B (n = 5). Differences between group 1 and 2B as well as 2A and 2B were statistically significant ( p = 0.025). No adverse effects were seen. All methods described were capable of creating new bone tissue with sufficient stability for successful implant placement.
2006
12
2649
2656
Two techniques for the preparation of cell-scaffold constructs suitable for sinus augmentation: steps into clinical application / Ingo N., Springer; Pier F., Nocini; Karl A., Schlegel; Daniele De, Santis; Jung, Park; Patrick H., Warnke; Hendrik, Terheyden; Robert, Zimmermann; Chiarini, Luigi; Klaus, Gardner; Francesca, Ferrari; Jörg, Wiltfang. - In: TISSUE ENGINEERING. - ISSN 1076-3279. - STAMPA. - 12:(2006), pp. 2649-2656. [10.1089/ten.2006.12.2649]
Ingo N., Springer; Pier F., Nocini; Karl A., Schlegel; Daniele De, Santis; Jung, Park; Patrick H., Warnke; Hendrik, Terheyden; Robert, Zimmermann; Chiarini, Luigi; Klaus, Gardner; Francesca, Ferrari; Jörg, Wiltfang
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/597327
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