Identification of differential photosynthetic-related changes in response to temperature and light intensity in a ‘winter’ and ‘spring’ barley genotype and in the derived segregating doubled-haploid population

The winter cold-resistant parent 'Nure' is an Italian two-rowed feeding barley cultivar, while the spring and cold-sensitive parent 'Tremois' is a French two-rowed malting variety. The response of the two genotypes have been characterised under several conditions of acclimation and stress to abiotic factors (chilling and freezing temperature, high light intensity, high temperature) using chlorophyll fluorescence and gas exchange analysis. The genotypes were contrasting not only for different levels of stress tolerance acquired after acclimation but also for a different capacity to induce earlier responses to stress conditions; this was especially evident in variation of chlorophyll fluorescence parameters at high light intensity associated to acclimation temperature and in different sugars accumulation in leaves and roots at low temperature.A segregating population of doubled haploid (DH) lines was derived by anther culture from the F1 generation of the 'winter' x 'spring' barley cross 'Nure' x 'Tremois', and a first analysis of a total of 136 Doubled-Haploid lines has been carried out.