The function of the 5' flanking region of the human elastin gene on transcription regulation has been investigated in chick embryo aorta cells by transient DNA transfer experiments with elastin-chloramphenicol acetyltransferase (CAT) fusions. The results have shown that the region comprised within -129 and -12 bp from the translation start site is essential for transcription and probably contains different control sequences. Expression of the reporter gene was increased 2-4-fold by addition of TGF-beta to the cell cultures. Analysis of CAT expression from different deletion constructs suggests that sequences in the region -196 to -12 play a major role in TGF-beta induction. The stimulating effect of the growth factor could not be observed when transfections were performed with chick embryo tendon fibroblasts. This suggests that transcriptional regulation of elastin by TGF-beta is tissue specific.

Regulation of the human elastin promoter in chick embryo cells. Tissue-specific effect of TGF-beta / Marigo, Valeria; Volpin, D.; Bressan, G. M.. - In: BIOCHIMICA ET BIOPHYSICA ACTA. - ISSN 0006-3002. - STAMPA. - 1172:(1993), pp. 31-36.

Regulation of the human elastin promoter in chick embryo cells. Tissue-specific effect of TGF-beta.

MARIGO, Valeria;
1993

Abstract

The function of the 5' flanking region of the human elastin gene on transcription regulation has been investigated in chick embryo aorta cells by transient DNA transfer experiments with elastin-chloramphenicol acetyltransferase (CAT) fusions. The results have shown that the region comprised within -129 and -12 bp from the translation start site is essential for transcription and probably contains different control sequences. Expression of the reporter gene was increased 2-4-fold by addition of TGF-beta to the cell cultures. Analysis of CAT expression from different deletion constructs suggests that sequences in the region -196 to -12 play a major role in TGF-beta induction. The stimulating effect of the growth factor could not be observed when transfections were performed with chick embryo tendon fibroblasts. This suggests that transcriptional regulation of elastin by TGF-beta is tissue specific.
1993
1172
31
36
Regulation of the human elastin promoter in chick embryo cells. Tissue-specific effect of TGF-beta / Marigo, Valeria; Volpin, D.; Bressan, G. M.. - In: BIOCHIMICA ET BIOPHYSICA ACTA. - ISSN 0006-3002. - STAMPA. - 1172:(1993), pp. 31-36.
Marigo, Valeria; Volpin, D.; Bressan, G. M.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/455499
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