Breast cancer is the most frequent neoplasm and the leading cause of cancer mortality in women worldwide. Metastasis to the axillary lymph nodes through the lymphatic vessels is a common step in the progression of breast cancer and an important prognostic factor. Moreover, metastatic involvement is the basis for surgical treatment of axillary lymph nodes. In order to seek for gene expression patterns associated with lymph node metastasis in primary breast cancer, we compared the transcriptional profiles of 20 radically resected primary breast cancers from patients without lymph node metastasis with those of 19 primary breast cancers with lymph node metastasis using high-density oligonucleotide microarrays (Affymetrix). Transcriptional data indicates that samples with lymph node metastasis can be distinguished from patients without metastasis and constitute a cluster with a clearly characterized gene expression fingerprint. A supervised analysis of the 39 cases stratified into 2 classes based on lymph-nodal metastasis identified 46 differentially expressed probe sets. In particular, ANOVA technique allowed selecting only those probes correlated to metastasis correcting for the estrogen receptor factor. The group of metastatic samples resulted in a well defined entity showing a distinctive gene expression pattern characterized by the over-expression of 23 probes, representing genes mainly involved in cell adhesion. Conversely, cancers from patients without lymph node metastasis presented the over-expression of transcripts related to protein transport, processing, and metabolism.Our data provide insights into the molecular and biological features of breast cancer development and progression. In addition, the differentially expressed genes identified may represent prognostic molecular markers of potential clinical relevance.

Lymph node metastatic fingerprint revealed by genome-wide transcriptional profiling of primary breast cancer / Belluco, C; Bicciato, Silvio; Bronte, V; Mandruzzato, S; Mammano, E; Marconato, G; Callegaro, A; Digito, M; Lise, M; Nitti, D.. - In: ANNALS OF SURGICAL ONCOLOGY. - ISSN 1068-9265. - STAMPA. - 13:(2006), pp. 26-26. (Intervento presentato al convegno 59th Annual Cancer Symposium of the Society of Surgical Oncology tenutosi a San Diego (CA, USA) nel March 23-26, 2006).

Lymph node metastatic fingerprint revealed by genome-wide transcriptional profiling of primary breast cancer

BICCIATO, Silvio;
2006

Abstract

Breast cancer is the most frequent neoplasm and the leading cause of cancer mortality in women worldwide. Metastasis to the axillary lymph nodes through the lymphatic vessels is a common step in the progression of breast cancer and an important prognostic factor. Moreover, metastatic involvement is the basis for surgical treatment of axillary lymph nodes. In order to seek for gene expression patterns associated with lymph node metastasis in primary breast cancer, we compared the transcriptional profiles of 20 radically resected primary breast cancers from patients without lymph node metastasis with those of 19 primary breast cancers with lymph node metastasis using high-density oligonucleotide microarrays (Affymetrix). Transcriptional data indicates that samples with lymph node metastasis can be distinguished from patients without metastasis and constitute a cluster with a clearly characterized gene expression fingerprint. A supervised analysis of the 39 cases stratified into 2 classes based on lymph-nodal metastasis identified 46 differentially expressed probe sets. In particular, ANOVA technique allowed selecting only those probes correlated to metastasis correcting for the estrogen receptor factor. The group of metastatic samples resulted in a well defined entity showing a distinctive gene expression pattern characterized by the over-expression of 23 probes, representing genes mainly involved in cell adhesion. Conversely, cancers from patients without lymph node metastasis presented the over-expression of transcripts related to protein transport, processing, and metabolism.Our data provide insights into the molecular and biological features of breast cancer development and progression. In addition, the differentially expressed genes identified may represent prognostic molecular markers of potential clinical relevance.
2006
13
26
26
Belluco, C; Bicciato, Silvio; Bronte, V; Mandruzzato, S; Mammano, E; Marconato, G; Callegaro, A; Digito, M; Lise, M; Nitti, D.
Lymph node metastatic fingerprint revealed by genome-wide transcriptional profiling of primary breast cancer / Belluco, C; Bicciato, Silvio; Bronte, V; Mandruzzato, S; Mammano, E; Marconato, G; Callegaro, A; Digito, M; Lise, M; Nitti, D.. - In: ANNALS OF SURGICAL ONCOLOGY. - ISSN 1068-9265. - STAMPA. - 13:(2006), pp. 26-26. (Intervento presentato al convegno 59th Annual Cancer Symposium of the Society of Surgical Oncology tenutosi a San Diego (CA, USA) nel March 23-26, 2006).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/421616
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