With the aim to detect what kind of cells; in addition to erythroid progenitors, could be involved in the pathogenesis of B 19 infection in some connective tissue diseases, primary Cultures of human fibroblasts (HF) and endothelial cells (HUVEC) were exposed to a B 19 positive serum (350 genome copies/cell). The presence of NS1 and VP1 mRNA, in both HF and HUVEC Cultures 1, 2 and 6 days after the exposure, indicated infection by B 19 virus. However, no significant increase of B 19 DNA level in the infected H[F and HUVEC cultures was detectable through the entire incubation period of 6 days. It is possible that HF and HUVEC are not permissive for B 19 virus replication or, alternatively, that few cells only get infected by B 19 virus. HF and HUVEC stimulation with different growth factors, or cytokines could be required for a B 19 productive infection to occur.

Human parvovirus B19 experimental infection in human fibroblasts and endothelial cells cultures / Zakrzewska, K; Cortivo, R; Tonello, C; Panfilo, S; Abatangelo, G; Giuggioli, D; Ferri, Clodoveo; Corcioli, F; Azzi, A.. - In: VIRUS RESEARCH. - ISSN 0168-1702. - STAMPA. - 114:1-2(2005), pp. 1-5. [10.1016/j.virusres.2005.05.003]

Human parvovirus B19 experimental infection in human fibroblasts and endothelial cells cultures

Giuggioli D;FERRI, Clodoveo;
2005

Abstract

With the aim to detect what kind of cells; in addition to erythroid progenitors, could be involved in the pathogenesis of B 19 infection in some connective tissue diseases, primary Cultures of human fibroblasts (HF) and endothelial cells (HUVEC) were exposed to a B 19 positive serum (350 genome copies/cell). The presence of NS1 and VP1 mRNA, in both HF and HUVEC Cultures 1, 2 and 6 days after the exposure, indicated infection by B 19 virus. However, no significant increase of B 19 DNA level in the infected H[F and HUVEC cultures was detectable through the entire incubation period of 6 days. It is possible that HF and HUVEC are not permissive for B 19 virus replication or, alternatively, that few cells only get infected by B 19 virus. HF and HUVEC stimulation with different growth factors, or cytokines could be required for a B 19 productive infection to occur.
2005
114
1-2
1
5
Human parvovirus B19 experimental infection in human fibroblasts and endothelial cells cultures / Zakrzewska, K; Cortivo, R; Tonello, C; Panfilo, S; Abatangelo, G; Giuggioli, D; Ferri, Clodoveo; Corcioli, F; Azzi, A.. - In: VIRUS RESEARCH. - ISSN 0168-1702. - STAMPA. - 114:1-2(2005), pp. 1-5. [10.1016/j.virusres.2005.05.003]
Zakrzewska, K; Cortivo, R; Tonello, C; Panfilo, S; Abatangelo, G; Giuggioli, D; Ferri, Clodoveo; Corcioli, F; Azzi, A.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/3116
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